Association of p53 codon 72 polymorphism with advanced lung cancer: the Arg allele is preferentially retained in tumours arising in Arg/Pro germline heterozygotes.
Association of p53 codon 72 polymorphism with advanced lung cancer: the Arg allele is preferentially retained in tumours arising in Arg/Pro germline heterozygotes.
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DOI：
10.1038/sj.bjc.6600595
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发表时间：
2002-10-21
影响因子：
8.8
通讯作者：
Spandidos, DA
中科院分区：
文献类型：
--
作者：
Papadakis, ED;Soulitzis, N;Spandidos, DA
p53密码子72多态性与癌症的关联已经由几个科学小组进行了研究，结果存在争议。在本研究中，我们研究了这种多态性的基因型频率在54例晚期肺癌患者和99名正常对照从希腊的地理区域。从每个病人的痰液和支气管冲洗样品进行了分析，人乳头瘤病毒的存在。还分析了密码子72杂合（Arg/Pro）患者在TP 53基因座处的杂合性缺失，以确定缺失的p53等位基因（Arg或Pro）。肺癌组p53 Arg/Arg基因型频率为50%，明显高于正常对照组的24.2%（P<0.002）。仅在2例患者（3.7%）中检测到人乳头瘤病毒。27例Arg/Pro患者中有14例（51.85%）出现TP 53基因座杂合性缺失。Pro等位基因丢失11例（78.6%），Arg等位基因丢失3例（21.4%）。我们的研究结果表明，p53密码子72精氨酸纯合性与晚期肺癌，精氨酸等位基因是优先保留在患者杂合子为这种多态性。另一方面，人乳头瘤病毒感染似乎并没有发挥重要作用，在肺癌的发生。英国癌症杂志（2002）87，1013-1018。www.bjcancer.com © 2002英国癌症研究中心
The association of p53 codon 72 polymorphism with cancer has been investigated by several scientific groups with controversial results. In the present study, we examined the genotypic frequency of this polymorphism in 54 patients with advanced lung cancer and 99 normal controls from the geographical region of Greece. Sputum and bronchial washing samples from each patient were assayed for the presence of human papillomavirus. Codon 72 heterozygous (Arg/Pro) patients were also analysed for loss of heterozygosity at the TP53 locus, in order to determine the lost p53 allele (Arg or Pro). p53 Arg/Arg genotype was significantly increased in lung cancer patients compared to normal controls (50% vs 24.2%, P<0.002). Human papillomavirus was detected only in two patients (3.7%). Loss of heterozygosity at the TP53 locus was found in 14 out of 27 Arg/Pro patients (51.85%). The Pro allele was lost in 11 cases (78.6%), while the Arg allele was lost in three (21.4%). Our results suggest that p53 codon 72 Arg homozygosity is associated with advanced lung cancer, and that the Arg allele is preferentially retained in patients heterozygous for this polymorphism. On the other hand, human papillomavirus infection does not seem to play an important role in lung carcinogenesis. British Journal of Cancer (2002) 87, 1013–1018. doi:10.1038/sj.bjc.6600595 www.bjcancer.com © 2002 Cancer Research UK
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影响因子：
2.4
作者：
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通讯作者：
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影响因子：
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DOI：
10.1165/ajrcmb.18.2.3033
发表时间：
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