Serum proteomics analysis and comparisons using iTRAQ in the progression of hepatitis B.
Serum proteomics analysis and comparisons using iTRAQ in the progression of hepatitis B.
复制标题
使用 iTRAQ 在乙型肝炎进展中进行血清蛋白质组学分析和比较。
DOI：
10.3892/etm.2013.1310
复制
发表时间：
2013-11
影响因子：
2.7
通讯作者：
Gao ZL
中科院分区：
文献类型：
--
作者：
Peng L;Liu J;Li YM;Huang ZL;Wang PP;Gu YR;Zheng YB;Gao ZL
本研究的目的是使用同量异位素标签相对和绝对定量（iTRAQ）分析法分析B型肝炎进展过程中血清蛋白水平的变化，并比较慢性B型肝炎（CH B）患者、B型肝炎病毒诱导的慢加急性肝衰竭（HBV诱导的ACLF）患者和正常人的血清蛋白水平。使用iTRAQ对15份血清样本进行蛋白质分析。研究人群包括健康对照组（n=5）、CHB患者（n=5）和HBV诱导的ACLF患者（n=5）。使用Western印迹法验证来自健康对照、CHB患者和HBV诱导的ACLF患者的另外9个血清样品（分别n=3）中的结果。使用iTRAQ分析，与健康对照相比，CHB和ACLF患者中鉴定了16种表达水平差异≥1.5倍的不同血清蛋白。其中5种蛋白质，C-反应蛋白前体，血红蛋白β链变体Hb S-Wake，载脂蛋白J前体，血小板因子4前体和玻连蛋白，其表达水平差异最大，与肝脏疾病的相关性最显著，随后使用蛋白质印迹法进行了验证。Western印迹结果与iTRAQ的结果一致。其中2个蛋白质未按生物学过程分类，所有蛋白质在HBV诱导的ACLF中的生物学功能尚不清楚。初步研究表明，HBV引起的各种血清蛋白的表达与不同的病理状态之间可能存在相关性。需要对大量样本进行分析，以确定可能参与B型肝炎发病机制和进展的潜在蛋白质生物标志物。
The aim of this study was to analyze the changes in serum protein levels in the progression of hepatitis B using isobaric tags for relative and absolute quantitation (iTRAQ) analysis, in addition to comparing the serum protein levels of patients with chronic hepatitis B (CHB), patients with hepatitis B virus-induced acute-on-chronic liver failure (HBV-induced ACLF) and normal individuals. Protein analysis was performed on 15 serum samples using iTRAQ. The study population included healthy controls (n=5), patients with CHB (n=5) and patients with HBV-induced ACLF (n=5). Western blotting was used to verify the results in an additional nine serum samples from healthy controls, patients with CHB and patients with HBV-induced ACLF (n=3, respectively). Using iTRAQ analysis, 16 different serum proteins with ≥1.5-fold differences in expression levels were identified in the patients with CHB and ACLF compared with the healthy controls. Five of those proteins, C-reactive protein precursor, hemoglobin β chain variant Hb S-Wake, apolipoprotein J precursor, platelet factor 4 precursor and vitronectin, which demonstrated the greatest differences in their expression levels and the most significant correlation with liver diseases, were subsequently verified using western blotting. The western blotting results were consistent with the results from the iTRAQ. Two of the five proteins are not classified by biological process, and the biological functions of all the proteins in HBV-induced ACLF remain unclear. This preliminary study demonstrated that a correlation between the expression of various serum proteins and the different pathogenetic conditions induced by HBV may exist. The analysis of a larger number of samples is required to identify potential protein biomarkers that may be involved in the pathogenesis and progression of hepatitis B.
登录
查看更多内容
影响因子：
3.3
作者：
Koukoulis, GK;Shen, JK;Gould, VE
通讯作者：
Gould, VE
影响因子：
3.1
作者：
Nahum, Elhanan;Livni, Gilat;Dagan, Ovadia
通讯作者：
Dagan, Ovadia
影响因子：
2.9
作者：
Poon, Terence C. W.;Pang, Ronald T. K.;Lo, Y. M. Dennis
通讯作者：
Lo, Y. M. Dennis
影响因子：
3.3
作者：
Kashiwagi, Manabu;Tanaka, Atsushi;Akasaka, Takashi
通讯作者：
Akasaka, Takashi
影响因子：
13.5
作者：
KOBAYASHI, J;YAMADA, S;KAWASAKI, H
通讯作者：
KAWASAKI, H