Pathogen-secreted proteases activate a novel plant immune pathway.
Pathogen-secreted proteases activate a novel plant immune pathway.
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DOI：
10.1038/nature14243
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发表时间：
2015-05-14
期刊：
影响因子：
64.8
通讯作者：
Ausubel, Frederick M.
中科院分区：
文献类型：
--
作者：
Cheng, Zhenyu;Li, Jian-Feng;Niu, Yajie;Zhang, Xue-Cheng;Woody, Owen Z.;Xiong, Yan;Djonovic, Slavica;Millet, Yves;Bush, Jenifer;McConkey, Brendan J.;Sheen, Jen;Ausubel, Frederick M.
丝裂原活化蛋白激酶（MAPK）级联在动植物的先天免疫信号网络中起着重要作用。然而，在植物中，信号感知如何被转导为MAPK激活的分子机制仍然难以捉摸。我们报道了病原体分泌的蛋白酶激活了拟南芥中一个以前未知的信号通路，涉及异源三聚体G蛋白复合物的Gα，Gβ和Gγ亚基，这些亚基在MAPK级联的上游起作用。在该途径中，活化C激酶1受体（RACK 1）作为一种新型支架发挥作用，与Gβ亚基以及MAPK级联的所有三级结合，从而将上游G蛋白信号传导与下游MAPK级联的活化联系起来。在这些研究中鉴定的蛋白酶-G蛋白-RACK 1-MAPK级联模块不同于先前描述的植物免疫信号传导途径，例如由细菌鞭毛蛋白引起的植物免疫信号传导途径，其中G蛋白在MAPK级联的下游或平行发挥作用，而不涉及RACK 1支架蛋白。发现新的蛋白酶介导的免疫信号传导途径，这里描述的是促进了广泛的主机范围，机会致病菌铜绿假单胞菌的使用。铜绿假单胞菌感染植物和动物的能力使其成为鉴定新型免疫调节策略的极好模型，所述免疫调节策略解释了其对不同宿主组织和免疫系统的生态位适应。
Mitogen-Activated Protein Kinase (MAPK) cascades play central roles in innate immune signaling networks in plants and animals. In plants, however, the molecular mechanisms of how signal perception is transduced to MAPK activation remain elusive. We report that pathogen-secreted proteases activate a previously unknown signaling pathway in Arabidopsis thaliana involving the Gα, Gβ and Gγ subunits of heterotrimeric G-protein complexes, which function upstream of a MAPK cascade. In this pathway, Receptor for Activated C Kinase 1 (RACK1) functions as a novel scaffold that binds to the Gβ subunit as well as to all three tiers of the MAPK cascade, thereby linking upstream G protein signaling to downstream activation of a MAPK cascade. The protease-G protein-RACK1-MAPK cascade modules identified in these studies are distinct from previously described plant immune signaling pathways such as the one elicited by bacterial flagellin, in which G proteins function downstream of or in parallel to a MAPK cascade without the involvement of the RACK1 scaffolding protein. The discovery of the novel protease-mediated immune signaling pathway described here was facilitated by the use of the broad host range, opportunistic bacterial pathogen Pseudomonas aeruginosa. The ability of P. aeruginosa to infect both plants and animals makes it an excellent model to identify novel types of immunoregulatory strategies that account for its niche adaptation to diverse host tissues and immune systems.
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影响因子：
5.1
作者：
Li JF;Park E;von Arnim AG;Nebenführ A
通讯作者：
Nebenführ A
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
11.6
作者：
Nakashima, Ayako;Chen, Letian;Shimamoto, Ko
通讯作者：
Shimamoto, Ko
影响因子：
6.9
作者：
Chen, Jin-Gui;Ullah, Hemayet;Jones, Alan M.
通讯作者：
Jones, Alan M.
影响因子：
11.6
作者：
Li, Jian-Feng;Chung, Hoo Sun;Sheen, Jen
通讯作者：
Sheen, Jen