A simple and rapid determination method for zooxanthellal genetic diversity in giant clams using multiplex PCR
A simple and rapid determination method for zooxanthellal genetic diversity in giant clams using multiplex PCR
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一种简单快速的多重PCR测定巨蛤虫黄藻遗传多样性的方法
DOI：
10.1007/s12562-016-1004-x
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发表时间：
2016
影响因子：
1.9
通讯作者：
Kazuhiko Koike
中科院分区：
文献类型：
--
作者：
Shota Ikeda;Hiroshi Yamashita;Lawrence M. Liao;Kazuhiko Koike
巨蛤（三纲贝类）是濒危物种，是重要的渔业资源。它们通过接受这些共生藻类的光合产物与虫黄藻（Symbiodinium）建立了共生关系。共生体分为不同的类群（A-I）；每一个生理性状归因于某一种可能是不同的，并可能影响感染蛤。然而，由于缺乏与巨蛤共生多样性相关的信息，导致巨蛤的培养困难，特别是在人工感染巨蛤幼虫的过程中，导致建立感染的成功率极低。在本报告中，我们建立了一种多重PCR方法，适用于分析巨蛤的共生伞型。我们设计了新的特异性PCR引物组，用于三种种型（A， C和D），它们具有不同的产物大小，因此在电泳凝胶上可以相互区分。该方法可靠，灵敏度高，适用于水产养殖。我们还进行了从蛤中提取共生体dna的试验案例，发现即使在同一池塘中饲养的蛤也存在意想不到的种型异质性。
Giant clams (tridacnid shellfishes) contain threatened species and represent important fishery resources. They establish a symbiotic relationship with zooxanthellae (Symbiodinium) by receiving photosynthetic products from these symbiotic algae.Symbiodiniumare divided into various phylotypes (A–I); each physiological character attributed to a certain phylotype may be different and may affect infectivity with clams. However, lack of information related toSymbiodiniumdiversities in giant clams causes difficulties in the culture of these clams, especially during the artificial infection process ofSymbiodiniumsources to clam larvae, which leads to an extremely low success rate in establishing infection. In this report, we have developed a multiplex PCR method, suitable for analyzingSymbiodiniumphylotypes in giant clams. We have designed new specific PCR primer sets for three phylotypes (A, C and D) that give different product sizes and thus are distinguishable from each other on an electrophoresis gel. The method is reliable, highly sensitive and most practicable for aquaculture using one PCR reaction. We also performed a trial case of the method for extractedSymbiodiniumDNA from clams and revealed an unexpected heterogeneity of phylotypes even though the clams tested were kept in the same pond.
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影响因子：
4.5
作者：
BECKVAR, N
通讯作者：
BECKVAR, N
影响因子：
2.5
作者：
Belda-Baillie, CA;Leggat, W;Yellowlees, D
通讯作者：
Yellowlees, D
影响因子：
1.6
作者：
Kinzie, RA;Takayama, M;Coffroth, MA
通讯作者：
Coffroth, MA
影响因子：
3.7
作者：
Kurihara T;Yamada H;Inoue K;Iwai K;Hatta M
通讯作者：
Hatta M
从某些太平洋砗磲科中分离出的虫黄藻的特性
影响因子：
2.9
作者：
D. L. Taylor
通讯作者：
D. L. Taylor