Impediment to symbiosis establishment between giant clams and Symbiodinium algae due to sterilization of seawater.
Impediment to symbiosis establishment between giant clams and Symbiodinium algae due to sterilization of seawater.
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DOI：
10.1371/journal.pone.0061156
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Hatta M
中科院分区：
文献类型：
--
作者：
Kurihara T;Yamada H;Inoue K;Iwai K;Hatta M
为了在幼年期生存，巨蛤幼体需要与环境中存在的微藻共生关系建立共生关系。巨蛤幼体成功建立共生关系的百分比（“共生率”）通常很低，这对种子生产者来说是个问题。我们研究了共生率如何以及为什么会发生变化，这取决于巨蛤种子是在经过紫外线处理还是未经紫外线处理的海水中连续饲养。结果反复表明，紫外线处理海水的共生率低于未经处理的海水。蒸压海水和0.2µm过滤海水的共生率也低于未经处理的海水。各种灭菌海水中共生率的降低说明在天然海水中帮助共生建立的某些因素可能因灭菌而减弱。可能的因素包括巨蛤种子的活力，因为额外的实验表明，在消毒海水中单独饲养的种子存活率低于未经处理的海水。综上所述，海水的紫外线处理导致共生率下降，这可能是由于各种灭菌技术共同的一些不利影响，并与巨蛤种子的活力有关。
To survive the juvenile stage, giant clam juveniles need to establish a symbiotic relationship with the microalgae Symbiodinium occurring in the environment. The percentage of giant clam juveniles succeeding in symbiosis establishment (“symbiosis rate”) is often low, which is problematic for seed producers. We investigated how and why symbiosis rates vary, depending on whether giant clam seeds are continuously reared in UV treated or non treated seawater. Results repeatedly demonstrated that symbiosis rates were lower for UV treated seawater than for non treated seawater. Symbiosis rates were also lower for autoclaved seawater and 0.2-µm filtered seawater than for non treated seawater. The decreased symbiosis rates in various sterilized seawater suggest the possibility that some factors helping symbiosis establishment in natural seawater are weakened owing to sterilization. The possible factors include vitality of giant clam seeds, since additional experiments revealed that survival rates of seeds reared alone without Symbiodinium were lower in sterilized seawater than in non treated seawater. In conclusion, UV treatment of seawater was found to lead to decreased symbiosis rates, which is due possibly to some adverse effects common to the various sterilization techniques and relates to the vitality of the giant clam seeds.
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影响因子：
64.8
作者：
Croft, MT;Lawrence, AD;Smith, AG
通讯作者：
Smith, AG
影响因子：
4.5
作者：
HESLINGA, GA;PERRON, FE;ORAK, O
通讯作者：
ORAK, O
影响因子：
4.5
作者：
CRAWFORD, CM;NASH, WJ;LUCAS, JS
通讯作者：
LUCAS, JS
影响因子：
1.6
作者：
FITT, WK;TRENCH, RK
通讯作者：
TRENCH, RK
DOI：
10.1016/0022-0981(95)00044-r
发表时间：
1995-08-10
影响因子：
2
作者：
HAWKINS, AJS;KLUMPP, DW
通讯作者：
KLUMPP, DW