Expression of TRPC6 channels in human epithelial breast cancer cells.
Expression of TRPC6 channels in human epithelial breast cancer cells.
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DOI：
10.1186/1471-2407-8-125
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发表时间：
2008-05-02
期刊：
影响因子：
3.8
通讯作者：
Ouadid-Ahidouch H
中科院分区：
文献类型：
--
作者：
Guilbert A;Dhennin-Duthille I;Hiani YE;Haren N;Khorsi H;Sevestre H;Ahidouch A;Ouadid-Ahidouch H
TRP通道已被证明参与肿瘤生成和恶性生长。然而，这些通道在乳腺癌中的表达仍不清楚。在这里，我们研究了内源性TRPC 6通道在乳腺癌细胞系（MCF-7），人乳腺癌上皮原代培养（hBCE）和正常和肿瘤乳腺组织中的表达和功能。分子生物学（Western blot和RT-PCR）和免疫组织化学技术用于研究TRPC 6的表达。为了研究MCF-7细胞和hBCE中的通道活性，我们使用电生理技术（全细胞膜片钳配置）。在hBCE和MCF-7细胞中，油酰基-2-乙酰基-sn-甘油（OAG）激活非选择性阳离子电流。2-APB、SK&F 96365和La ~（3+）可抑制OAG内向电流。TRPC 6在hBCE和MCF-7细胞中均表达，而TRPM 7则不表达。TRPC 3仅在hBCE中表达。临床上，TRPC 6 mRNA和蛋白质在乳腺癌标本中与正常乳腺组织相比升高。此外，我们发现TRPC 6蛋白水平的过表达与肿瘤分级、雌激素受体表达或淋巴结阳性肿瘤无关。我们的研究结果表明，TRPC 6通道在乳腺癌上皮细胞中强烈表达和功能。此外，这些通道的过度表达似乎与肿瘤分级、ER表达和淋巴结转移无关。我们的研究结果支持TRPC 6可能在乳腺癌发生中发挥作用的观点。
TRP channels have been shown to be involved in tumour generation and malignant growth. However, the expression of these channels in breast cancer remains unclear. Here we studied the expression and function of endogenous TRPC6 channels in a breast cancer cell line (MCF-7), a human breast cancer epithelial primary culture (hBCE) and in normal and tumour breast tissues. Molecular (Western blot and RT-PCR), and immunohistochemical techniques were used to investigate TRPC6 expression. To investigate the channel activity in both MCF-7 cells and hBCE we used electrophysiological technique (whole cell patch clamp configuration). A non selective cationic current was activated by the oleoyl-2-acetyl-sn-glycerol (OAG) in both hBCE and MCF-7 cells. OAG-inward current was inhibited by 2-APB, SK&F 96365 and La3+. TRPC6, but not TRPM7, was expressed both in hBCE and in MCF-7 cells. TRPC3 was only expressed in hBCE. Clinically, TRPC6 mRNA and protein were elevated in breast carcinoma specimens in comparison to normal breast tissue. Furthermore, we found that the overexpression of TRPC6 protein levels were not correlated with tumour grades, estrogen receptor expression or lymph node positive tumours. Our results indicate that TRPC6 channels are strongly expressed and functional in breast cancer epithelial cells. Moreover, the overexpression of these channels appears without any correlation with tumour grade, ER expression and lymph node metastasis. Our findings support the idea that TRPC6 may have a role in breast carcinogenesis.
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影响因子：
3.7
作者：
Sergeev, IN;Rhoten, WB
通讯作者：
Rhoten, WB
影响因子：
5.7
作者：
Potier, Marie;Joulin, Virginie;Vandier, Christophe
通讯作者：
Vandier, Christophe
影响因子：
20.1
作者：
Ng, LC;Gurney, AM
通讯作者：
Gurney, AM
影响因子：
8
作者：
Lehen'kyi, V.;Flourakis, M.;Prevarskaya, N.
通讯作者：
Prevarskaya, N.
DOI：
10.1006/bbrc.2001.4638
发表时间：
2001-04-06
影响因子：
3.1
作者：
Peng, JB;Zhuang, LY;Freeman, MR
通讯作者：
Freeman, MR