Differential gene expression in male and female rainbow trout embryos prior to the onset of gross morphological differentiation of the gonads.
Differential gene expression in male and female rainbow trout embryos prior to the onset of gross morphological differentiation of the gonads.
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DOI：
10.1186/1471-2164-12-404
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发表时间：
2011-08-08
期刊：
影响因子：
4.4
通讯作者：
Nichols KM
中科院分区：
文献类型：
--
作者：
Hale MC;Xu P;Scardina J;Wheeler PA;Thorgaard GH;Nichols KM
性别之间在遗传水平上有很大的差异；这些差异包括异性配对的性染色体和/或性别之间的基因表达差异。在虹鱼(Oncorhynchus MykISS)中，qRT-PCR研究发现几个候选性别决定基因的表达存在显著差异。然而，这些基因只占基因组的一小部分，其他物种的研究表明，有很大一部分转录组在性别之间存在差异表达。一旦性腺分化和成熟发生，这些差异就特别明显，但在发育的早期阶段就知之甚少。在这里，我们使用微阵列和qRT-PCR的数据来鉴定在孵化前胚胎的三个时间点性别之间差异表达的基因，在该物种已知的性别分化时间之前。微阵列研究揭示了883个性别之间的差异表达特征，其中男性和女性在不同时间点的上调特征数量大致相同。微阵列上的大多数差异表达基因与性功能无关，这表明性别之间的基因表达在发育早期就存在着大规模的差异。候选基因分析表明，Sox9、DMRT1、Nr5a1和WT1在雄性中表达上调，FOXL2、Ovol1、fst和cyp19a1a在雌性中表达上调。这是首次发现鱼类胚胎发生期间基因组表达的性别二型性，并证明在性腺发生完成之前就存在转录差异。
There are large differences between the sexes at the genetic level; these differences include heterogametic sex chromosomes and/or differences in expression of genes between the sexes. In rainbow trout (Oncorhynchus mykiss) qRT-PCR studies have found significant differences in expression of several candidate sex determining genes. However, these genes represent a very small fraction of the genome and research in other species suggests there are large portions of the transcriptome that are differentially expressed between the sexes. These differences are especially noticeable once gonad differentiation and maturation has occurred, but less is known at earlier stages of development. Here we use data from a microarray and qRT-PCR to identify genes differentially expressed between the sexes at three time points in pre-hatch embryos, prior to the known timing of sexual differentiation in this species. The microarray study revealed 883 differentially expressed features between the sexes with roughly equal numbers of male and female upregulated features across time points. Most of the differentially expressed genes on the microarray were not related to sex function, suggesting large scale differences in gene expression between the sexes are present early in development. Candidate gene analysis revealed sox9, DMRT1, Nr5a1 and wt1 were upregulated in males at some time points and foxl2, ovol1, fst and cyp19a1a were upregulated in females at some time points. This is the first study to identify sexual dimorphism in expression of the genome during embryogenesis in any fish and demonstrates that transcriptional differences are present before the completion of gonadogenesis.
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DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
30.8
作者：
Ayroles, Julien F.;Carbone, Mary Anna;Stone, Eric A.;Jordan, Katherine W.;Lyman, Richard F.;Magwire, Michael M.;Rollmann, Stephanie M.;Duncan, Laura H.;Lawrence, Faye;Anholt, Robert R. H.;Mackay, Trudy F. C.
通讯作者：
Mackay, Trudy F. C.
影响因子：
3.6
作者：
Baron, D;Houlgatte, R;Guiguen, Y
通讯作者：
Guiguen, Y
影响因子：
10.7
作者：
Mank, Judith E.;Nam, Kiwoong;Ellegren, Hans
通讯作者：
Ellegren, Hans
影响因子：
5.3
作者：
De Santa Barbara, P;Bonneaud, N;Berta, P
通讯作者：
Berta, P