Using simple donors to drive the equilibria of glycosyltransferase-catalyzed reactions.
Using simple donors to drive the equilibria of glycosyltransferase-catalyzed reactions.
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DOI：
10.1038/nchembio.638
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发表时间：
2011-08-21
影响因子：
14.8
通讯作者：
Thorson, Jon S.
中科院分区：
文献类型：
--
作者：
Gantt, Richard W.;Peltier-Pain, Pauline;Cournoyer, William J.;Thorson, Jon S.
We report the ability of simple glycoside donors to drastically shift the equilibria of glycosyltransferase-catalyzed reactions, transforming NDP-sugar formation from an endo- to an exothermic process. To demonstrate the utility of this thermodynamic adaptability, we highlight the glycosyltransferase-catalyzed synthesis of 22 sugar nucleotides from simple aromatic sugar donors as well as the corresponding in situ formation of sugar nucleotides as a driving force in context of glycosyltransferase-catalyzed reactions for small molecule glycodiversification. These simple aromatic donors also enabled the first general colorimetric assay for glycosyltransfer, applicable to drug discovery, protein engineering, and other fundamental sugar nucleotide-dependent investigations. This study directly challenges the general notion that NDP-sugars are ‘high-energy’ sugar donors when taken out of their traditional biological context.
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