De novo assembly, annotation, and comparative analysis of 26 diverse maize genomes.
De novo assembly, annotation, and comparative analysis of 26 diverse maize genomes.
复制标题
DOI：
10.1126/science.abg5289
复制
发表时间：
2021-08-06
期刊：
影响因子：
--
通讯作者：
Dawe RK
中科院分区：
文献类型：
--
作者：
Hufford MB;Seetharam AS;Woodhouse MR;Chougule KM;Ou S;Liu J;Ricci WA;Guo T;Olson A;Qiu Y;Della Coletta R;Tittes S;Hudson AI;Marand AP;Wei S;Lu Z;Wang B;Tello-Ruiz MK;Piri RD;Wang N;Kim DW;Zeng Y;O'Connor CH;Li X;Gilbert AM;Baggs E;Krasileva KV;Portwood JL 2nd;Cannon EKS;Andorf CM;Manchanda N;Snodgrass SJ;Hufnagel DE;Jiang Q;Pedersen S;Syring ML;Kudrna DA;Llaca V;Fengler K;Schmitz RJ;Ross-Ibarra J;Yu J;Gent JI;Hirsch CN;Ware D;Dawe RK
我们报告从头基因组组装，转录组，注释和甲基化的26个自交系，作为创始人的玉米巢式关联映射人口。在这些不同的基因组中，泛基因的数量超过103，000个，其中大约三分之一在所有基因型中发现。结果表明，玉米的古老四倍体性状继续通过分馏退化到今天。在重复阵列和完整的注释着丝粒上的优秀的连续性揭示了主要细胞学标志的额外变化。我们表明，结合结构变异与SNPs可以提高定量作图研究的能力。最后，我们记录了DNA甲基化水平的变异，并证明了未甲基化区域富含有助于表型变异的顺式调控元件。玉米的多基因组分析揭示了基因内容，基因组结构和甲基化的先前未知的变化。
We report de novo genome assemblies, transcriptomes, annotations, and methylomes for the 26 inbreds that serve as the founders for the maize nested association mapping population. The number of pan-genes in these diverse genomes exceeds 103,000, with approximately a third found across all genotypes. The results demonstrate that the ancient tetraploid character of maize continues to degrade by fractionation to the present day. Excellent contiguity over repeat arrays and complete annotation of centromeres revealed additional variation in major cytological landmarks. We show that combining structural variation with SNPs can improve the power of quantitative mapping studies. Finally, we document variation at the level of DNA methylation, and demonstrate that unmethylated regions are enriched for cis-regulatory elements that contribute to phenotypic variation. A multi-genome analysis of maize reveals previously unknown variation in gene content, genome structure, and methylation.
登录
查看更多内容
影响因子：
1.7
作者：
Albert, P. S.;Gao, Z.;Birchler, J. A.
通讯作者：
Birchler, J. A.
影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
影响因子：
4.5
作者：
Bilinski, Paul;Albert, Patrice S.;Ross-Ibarra, Jeffrey
通讯作者：
Ross-Ibarra, Jeffrey
影响因子：
48
作者：
Chin, Chen-Shan;Peluso, Paul;Schatz, Michael C.
通讯作者：
Schatz, Michael C.
影响因子：
30.8
作者：
Chia, Jer-Ming;Song, Chi;Ware, Doreen
通讯作者：
Ware, Doreen