Nanopore-based detection of circulating microRNAs in lung cancer patients.
Nanopore-based detection of circulating microRNAs in lung cancer patients.
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DOI：
10.1038/nnano.2011.147
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发表时间：
2011-09-04
影响因子：
38.3
通讯作者：
中科院分区：
文献类型：
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作者：
MicroRNA是调节基因表达的短RNA分子。由于它们的表达水平与各种疾病相关，因此已被研究为潜在的生物标志物。然而，血流中microRNA的检测仍然很困难，因为目前的方法没有足够的选择性或敏感性。在这里，我们展示了一种基于α-溶血素蛋白的纳米孔传感器在单分子水平上选择性地检测肺癌患者血浆样本中的microRNA，而无需标记或扩增。该传感器使用可编程的寡核苷酸探针来产生靶特异性特征信号，能够定量亚皮摩尔水平的癌症相关microRNA，并区分microRNA家族成员之间的单核苷酸差异。这种方法可以证明用于定量microRNA检测，生物标志物发现和癌症的非侵入性早期诊断。
MicroRNAs are short RNA molecules that regulate gene expression. They have been investigated as potential biomarkers because their expression levels are correlated with various diseases. However, the detection of microRNAs in the bloodstream remains difficult because current methods are not sufficiently selective or sensitive. Here, we show that a nanopore sensor based on the alpha-hemolysin protein selectively detected microRNAs at the single molecular level in plasma samples from lung cancer patients without the need for labelling or amplification. The sensor, which used a programmable oligonucleotide probe to generate a target-specific signature signal, was able to quantify sub-picomolar levels of cancer-associated microRNAs and to discriminate single nucleotide differences between microRNA family members. This approach could prove useful for quantitative microRNA detection, biomarker discovery, and the non-invasive early diagnosis of cancer.
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影响因子：
14.9
作者：
Kozomara A;Griffiths-Jones S
通讯作者：
Griffiths-Jones S
影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
--
作者：
Bayley H;Cronin B;Heron A;Holden MA;Hwang WL;Syeda R;Thompson J;Wallace M
通讯作者：
Wallace M
DOI：
10.1158/1078-0432.ccr-09-1736
发表时间：
2010-01-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Landi MT;Zhao Y;Rotunno M;Koshiol J;Liu H;Bergen AW;Rubagotti M;Goldstein AM;Linnoila I;Marincola FM;Tucker MA;Bertazzi PA;Pesatori AC;Caporaso NE;McShane LM;Wang E
通讯作者：
Wang E
影响因子：
64.5
作者：
Diederichs, Sven;Haber, Daniel A.
通讯作者：
Haber, Daniel A.