Toxicological Evaluation and Protective Effects of Ethanolic Leaf Extract of Cassia spectabilis DC on Liver and Kidney Function of Plasmodium berghei-Infected Mice.
Toxicological Evaluation and Protective Effects of Ethanolic Leaf Extract of Cassia spectabilis DC on Liver and Kidney Function of Plasmodium berghei-Infected Mice.
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DOI：
10.1155/2022/6770828
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发表时间：
2022
影响因子：
3.1
通讯作者：
Arwati H
中科院分区：
文献类型：
--
作者：
Ekasari W;Mahardiani A;Putri NT;Wahyuni TS;Arwati H
目前，抗疟药物耐药性的存在已成为疟疾治疗的主要障碍。为了克服这个问题，需要进行一系列的研究，从植物中找到新的抗疟药物。以前，已经报道了决明子叶的90%乙醇提取物（EECS）在体外对恶性疟原虫（Plasmodium falciparum）和体内对伯氏疟原虫（Plasmodium berghei ANKA）具有抗疟活性。本研究旨在探讨EECS对伯氏疟原虫ANKA感染小鼠肝脏和肾脏的毒性及保护作用。对健康小鼠进行急性和亚急性毒性试验，以150 mg/kg BW的剂量给予EECS。在感染伯氏疟原虫的小鼠中以150和200 mg/kg BW的剂量进行抗疟活性试验。关于肝肿大，进一步分析肝酶的血浆水平，以及肝脏的组织病理学观察，以确定提取物对肝脏的影响。同时对肾脏进行组织病理学观察。急性毒性试验结果表明，EECS在最高剂量下无小鼠死亡，对小鼠安全。基于组织学数据的亚急性毒性显示，试验组和健康组小鼠的肝脏和肾脏无显著差异。在感染伯氏疟原虫的小鼠中，EECS的组织学和酶效应显示出改善肝功能的组织学和酶效应以及对肾脏的组织病理学效应，与阴性对照相比，200 mg/kg BW剂量下的活性最高。结果表明，EECS对小鼠无毒性，并能修复伯氏疟原虫ANKA感染小鼠的肝肾功能，是一种良好的抗疟药物。
Currently, the presence of antimalarial drug resistance has become a major obstacle in the treatment of malaria. To overcome the problem, a series of studies are needed to find new antimalarial drugs from plants. Previously, 90% ethanolic extract of Cassia spectabilis DC (EECS) leaves have been reported to have antimalarial activity in vitro against Plasmodium falciparum and in vivo against Plasmodium berghei ANKA. The research is conducted to find out the toxicity and protective effects of EECS on the liver and kidneys of mice infected with P. berghei ANKA. The acute and subacute toxicity tests were carried out on healthy mice that were given EECS at a dose of 150 mg/kg BW. An antimalarial activity test was carried out at doses of 150 and 200 mg/kg BW in P. berghei-infected mice. Regarding hepatomegaly, further plasma levels of hepatic enzyme were analyzed, as well as histopathological observation of the liver to determine the effect of the extract on liver. The kidney was observed histopathologically as well. The acute toxicity test of EECS showed that there was no mouse died at the highest dose, indicating safe for the mice. The subacute toxicity based on the histology data showed no significant difference in the liver and kidney of mice between the tested group and the healthy group. The histological and enzymatic effect of EECS in mice infected with P. berghei showed the histological and enzymatic effect that improved liver function and the histopathological effect on kidneys with the highest activity at a dose of 200 mg/kg BW compared with the negative control. The results showed the EECS was not toxic in mice and repaired the liver and kidney functions of P. berghei ANKA-infected mice, indicating a good candidate for antimalarial drug development.
影响因子：
3.9
作者：
Ekasari W;Basuki DR;Arwati H;Wahyuni TS
通讯作者：
Wahyuni TS