Antiplasmodial activity of Ethanolic extract of Cassia spectabilis DC leaf and its inhibition effect in Heme detoxification.
Antiplasmodial activity of Ethanolic extract of Cassia spectabilis DC leaf and its inhibition effect in Heme detoxification.
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DOI：
10.1186/s12906-021-03239-9
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发表时间：
2021-02-19
影响因子：
3.9
通讯作者：
Wahyuni TS
中科院分区：
文献类型：
--
作者：
Ekasari W;Basuki DR;Arwati H;Wahyuni TS
在以往的研究中，决明子叶表现出良好的抗疟原虫活性。因此，本研究是对杜仲叶提取物的后续研究。研究表明，在体外和体内抗疟原虫活性和抗疟机制方面，将提取物分级、亚分级和分离以获得纯化的化合物。体外抗恶性疟原虫活性试验，寻找活性化合物。对伯氏疟原虫ANKA感染的小鼠进行体内试验，以确定单独或与青蒿琥酯组合的预防活性和抗疟原虫活性。使用Basilico方法进行作为抗疟机制之一的血红素解毒抑制试验。结果表明，从C.一种具有与（−）-7-羟基卡西碱相同的结构模式的紫堇叶。90%乙醇提取物的预防试验。结果表明，单用山茱萸DC叶对感染伯氏疟原虫ANKA的小鼠的抑菌率最高，为68.61%，而阳性对照（强力霉素13 mg/kg）为73.54%。与青蒿琥酯联合使用，C. 150 mg/kg，每日3次。复方青蒿琥酯（D_0-D_2）对小鼠的抑瘤作用优于阿莫地喹+青蒿琥酯，其抑瘤率分别为99.18%和92.88%。其抑制血红素解毒的IC_（50）为0.375 mg/ml，优于二磷酸氯喹（0.682 mg/ml）。C.结果表明，山茱萸叶具有较强的抗疟原虫活性，有望成为一种经济有效的抗疟植物药。
In previous studies, Cassia spectabilis DC leaf has shown a good antiplasmodial activity. Therefore, this study is a follow-up study of the extract of leaf of C. spectabilis DC on its in vitro and in vivo antiplasmodial activity and mechanism as an antimalarial. The extract was fractionated, sub-fractionated and isolated to obtain the purified compound. In vitro antiplasmodial activity test against Plasmodium falciparum to find out the active compound. In vivo test against P. berghei ANKA-infected mice was conducted to determine prophylactic activity and antiplasmodial activity either alone or in combination with artesunate. The inhibition of heme detoxification test as one of the antimalarial mechanisms was carried out using the Basilico method. The results showed that active antimalarial compound isolated from C. spectabilis DC leaf had a structural pattern that was identical to (−)-7-hydroxycassine. Prophylactic test of 90% ethanolic extract of C. spectabilis DC leaf alone against P. berghei ANKA-infected mice obtained the highest percentage inhibition was 68.61%, while positive control (doxycycline 13 mg/kg) was 73.54%. In combination with artesunate, 150 mg/kg three times a day of C. spectabilis DC (D0-D2) + artesunate (D2) was better than the standard combination of amodiaquine + artesunate where the inhibition percentages were 99.18 and 92.88%, respectively. The IC50 of the extract for the inhibitory activity of heme detoxification was 0.375 mg/ml which was better than chloroquine diphosphate (0.682 mg/ml). C. spectabilis DC leaf possessed potent antiplasmodial activity and may offer a potential agent for effective and affordable antimalarial phytomedicine.
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影响因子：
1.4
作者：
Viegas Junior, Cláudio;Pivatto, Marcos;Bolzani, Vanderlan da Silva
通讯作者：
Bolzani, Vanderlan da Silva
影响因子：
2.2
作者：
Somsak V;Borkaew P;Klubsri C;Dondee K;Bootprom P;Saiphet B
通讯作者：
Saiphet B
影响因子：
--
作者：
Elyazar, Iqbal R. F.;Hay, Simon I.;Baird, J. Kevin
通讯作者：
Baird, J. Kevin
影响因子：
64.8
作者：
SLATER, AFG;CERAMI, A
通讯作者：
CERAMI, A
影响因子：
5.4
作者：
Muñoz, V;Sauvain, M;Deharo, E
通讯作者：
Deharo, E