Reparative effects of neural stem cells in neonatal rats with hypoxic-ischemic injury are not influenced by host sex.
Reparative effects of neural stem cells in neonatal rats with hypoxic-ischemic injury are not influenced by host sex.
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DOI：
10.1038/pr.2014.7
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发表时间：
2014-05
影响因子：
3.6
通讯作者：
Obenaus, Andre
中科院分区：
文献类型：
--
作者：
Ashwal, Stephen;Ghosh, Nirmalya;Turenius, Christine I.;Dulcich, Melissa;Denham, Christopher M.;Tone, Beatriz;Hartman, Richard;Snyder, Evan Y.;Obenaus, Andre
越来越多的人认识到性别对大脑发育、疾病易感性和对药物/康复治疗的反应有重要影响。在再生医学中，移植的干细胞和宿主性别之间是否存在相互作用仍然是完全未知的，这种相互作用可能会使某些患者的疗效和安全性产生偏差，而对其他患者则不会。我们研究了受体性别在新生大鼠缺氧缺血性损伤（HII）模型中的作用，该模型用人类女性神经干细胞（hNSCs）处理，用超顺磁性氧化铁（SPIO）颗粒标记植入对侧脑室。我们监测HII进化（通过MRI、组织病理学、行为测试）和hNSC命运（迁移、复制、存活力）。植入后的性别不影响缺血损伤的体积或位置（1，30或90天）或行为（90天）。SPIO标记不影响HII的演变。植入对轻度/中度损伤的受益最大，这些损伤保持稳定，而不是像严重HII那样增加，这是此类病变的自然病程。我们的研究结果表明，hNSC治疗（包括使用预先标记有铁的hNSC，以允许通过MRI进行真实的跟踪）对于患有轻度至中度HII的男性和女性人类新生儿同样安全有效。
Gender is increasingly recognized as an important influence on brain development, disease susceptibility, and response to pharmacologic/rehabilitative treatments. In regenerative medicine, it remains entirely unknown whether there is an interaction between transplanted stem cells and host gender that might bias efficacy and safety in some patients but not others. We examined the role of recipient gender in a neonatal rat hypoxia-ischemic injury (HII) model, treated with human female neural stem cells (hNSCs), labeled with superparamagnetic iron-oxide (SPIO) particles implanted into the contralateral cerebral ventricle. We monitored HII evolution (by MRI, histopathology, behavioral testing) and hNSC fate (migration, replication, viability). Recipient gender after implantation did not influence the volume or location of ischemic injury (1, 30, or 90d) or behavior (90d). SPIO labeling did not influence HII evolution. Implantation had its greatest benefit on mild/moderate injuries which remained stable rather than increasing as in severe HII as is the natural history for such lesions. Our results suggest that hNSC treatment (including using hNSCs that are pre-labeled with iron to allow tracking in real time by MRI) would be equally safe and effective for male and female human newborns with mild-to-moderate HII.
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影响因子：
11.2
作者：
Obenaus, Andre;Dilmac, Nejmi;Tone, Beatriz;Tian, Hou Rou;Hartman, Richard;Digicaylioglu, Murat;Snyder, Evan Y.;Ashwal, Stephen
通讯作者：
Ashwal, Stephen
影响因子：
5.3
作者：
Park, Kook In;Himes, B. Timothy;Snyder, Evan Y.
通讯作者：
Snyder, Evan Y.
影响因子：
6.3
作者：
Recker, Rebecca;Adami, Arash;Ashwal, Stephen
通讯作者：
Ashwal, Stephen
影响因子：
11.2
作者：
Normann, Sandra;de Veber, Gabrielle;Nowak-Goettl, Ulrike
通讯作者：
Nowak-Goettl, Ulrike
影响因子：
3.8
作者：
Kang, Kyung-Sun;Trosko, James E.
通讯作者：
Trosko, James E.