Electron tomography on γ-aminobutyric acid-ergic synapses reveals a discontinuous postsynaptic network of filaments.
Electron tomography on γ-aminobutyric acid-ergic synapses reveals a discontinuous postsynaptic network of filaments.
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电子断层扫描在γ-氨基丁基酸性突触上揭示了细丝的突触后网络。
DOI：
10.1002/cne.23453
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发表时间：
2014-03
影响因子：
2.5
通讯作者：
Reese, Thomas S.
中科院分区：
文献类型：
--
作者：
Linsalata, Alexander E.;Chen, Xiaobing;Winters, Christine A.;Reese, Thomas S.
γ-氨基丁酸（GABA）能突触的突触强度调节依赖于GABA能突触后位点细胞质蛋白对GABA A型受体的动态捕获、保留和调节。这些蛋白质在突触后细胞质中是如何定向和组织的还没有确定。为了更好地了解这些结构，并进一步了解它们在突触后位点调节受体群体的机制，我们利用电子断层扫描检查GABA能突触在分离的大鼠海马培养物。GABA能突触被确定和选择断层扫描使用一组标准来自免疫金标记的GABA能突触的结构。体层摄影显示出一个复杂的突触后网络，由从突触后膜延伸到细胞质中约100 nm的细丝组成。这些突触后纤维的分布是惊人的相似的免疫金标记的桥蛋白。细丝通过均匀的接触模式相互连接，形成由2-12根细丝组成的复合体。复合物没有连接，形成一个完整的，连续的支架，表明GABA能突触后专业化是不严格的组织比神经元突触后密度。
The regulation of synaptic strength at γ-aminobutyric acid (GABA)-ergic synapses is dependent on the dynamic capture, retention, and modulation of GABA A-type receptors by cytoplasmic proteins at GABAergic postsynaptic sites. How these proteins are oriented and organized in the postsynaptic cytoplasm is not yet established. In order to better understand these structures and gain further insight into the mechanisms by which they regulate receptor populations at postsynaptic sites, we utilized electron tomography to examine GABAergic synapses in dissociated rat hippocampal cultures. GABAergic synapses were identified and selected for tomography using a set of criteria derived from the structure of immunogold-labeled GABAergic synapses. Tomography revealed a complex postsynaptic network composed of filaments that extend ∼100 nm into the cytoplasm from the postsynaptic membrane. The distribution of these postsynaptic filaments was strikingly similar to that of the immunogold label for gephyrin. Filaments were interconnected through uniform patterns of contact, forming complexes composed of 2-12 filaments each. Complexes did not link to form an integrated, continuous scaffold, suggesting that GABAergic postsynaptic specializations are less rigidly organized than glutamatergic postsynaptic densities.
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影响因子：
7.8
作者：
BANKER, G;CHURCHILL, L;COTMAN, CW
通讯作者：
COTMAN, CW
影响因子：
2.5
作者：
CABOT, JB;BUSHNELL, A;MENDELL, NR
通讯作者：
MENDELL, NR
影响因子：
3.5
作者：
Danglot, L;Triller, A;Bessis, A
通讯作者：
Bessis, A
DOI：
10.1083/jcb.74.1.181
发表时间：
1977-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cohen RS;Blomberg F;Berzins K;Siekevitz P
通讯作者：
Siekevitz P
影响因子：
3.3
作者：
Du, J;Tao-Cheng, JH;McBain, CJ
通讯作者：
McBain, CJ