Cloning and Characterization of a Novel Hypersensitive-induced Reaction Gene from Wheat Infected by Stripe Rust Pathogen
Cloning and Characterization of a Novel Hypersensitive-induced Reaction Gene from Wheat Infected by Stripe Rust Pathogen
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条锈菌感染小麦中新型过敏诱导反应基因的克隆和鉴定
DOI：
10.1111/j.1439-0434.2009.01556.x
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发表时间：
2009-12
影响因子：
1.5
通讯作者：
Dong, Yan-Ling
中科院分区：
文献类型：
--
作者：
Wang, Xiao-Jie;Zhang, Gang;Li, Yi-Min;Huang, Li-Li;Han, Qing-Mei;Kang, Zhen-Sheng;Zhang, Yi;Guo, Jun;Dong, Yan-Ling
过敏反应是植物抵抗病原体感染的最复杂和最有效的防御反应形式之一;它涉及感染部位及其周围的植物细胞的快速死亡，以及病原体生长的限制。基于先前从抗条锈小麦成株大规模转录谱中鉴定的差异表达转录衍生片段（其与大麦HvHIR 3基因高度相似），利用电子克隆鉴定了来自条锈病原体感染的小麦的新的过敏诱导反应（HIR）基因，命名为TaHIR 3，结合逆转录-聚合酶链反应（RT-PCR）方法。预计TaHIR 3编码一个287个氨基酸的蛋白质，推导分子量为31.5 kDa，pI为5.68。TaHIR 3的氨基酸序列分析表明存在Stomatins、Prohibitin、Flotillins、HflK/C蛋白结构域和Prohibitin同源物。系统发育分析表明，小麦TaHIR 3基因与大麦HvHIR 3基因在cDNA和推导的氨基酸水平上具有高度的同源性。利用实时荧光定量RT-PCR技术，分析了TaHIR 3基因在小麦成株期（不亲和期）和苗期（亲和期）感染条锈菌后的表达谱。结果表明，在成株期，TaHIR 3在接种后被显著诱导，诱导高峰出现在接种后48 h。在幼苗阶段，TaHIR 3也早在12 hpi上调。但其转录量相对低于成株期。
The hypersensitive response is one of the most sophisticated and efficient forms of plant defence response against fighting pathogen infection; it involves the rapid death of plant cells at and around infection sites, together with the restriction of pathogen growth. Based on a differentially expressed transcribed derived fragment previously identified from the large-scale transcript profiling of adult-plant wheat resistant to stripe rust - which was highly similar to the barley HvHIR3 gene - a novel hypersensitive-induced reaction (HIR) gene from wheat infected by stripe rust pathogen, designated as TaHIR3, was identified using in silico cloning, combined with reverse transcriptase-polymerase chain reaction (RT-PCR) method. TaHIR3 was predicted to encode a 287-amino acid protein with a deduced molecular weight of 31.5 kDa and a pI of 5.68. An amino acid sequence analysis of TaHIR3 demonstrated the presence of the Stomatins, Prohibitin, Flotillins, HflK/C protein domain and prohibitin homologues. A phylogenetic analysis of 17 HIR genes from different plant species indicated that the wheat TaHIR3 gene was highly homologous to the barley HvHIR3 gene, at both the cDNA and deduced amino acid levels. Using a real-time quantitative RT-PCR, expression profiles of TaHIR3 in wheat leaves that had been infected with stripe rust fungus at the adult-plant (incompatible) and seedling (compatible) stages were analysed. The results revealed that at the adult-plant stage, TaHIR3 was significantly induced after inoculation, with the occurrence maximum induction at 48 h postinoculation (hpi). At the seedling stage, TaHIR3 was also up-regulated as early as 12 hpi. However, the transcript amount was relatively lower than that of the adult-plant stage.
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影响因子：
1.1
作者：
Ogihara, Y;Mochida, K;Yamazaki, Y
通讯作者：
Yamazaki, Y
DOI：
--
发表时间：
2006
期刊：
Acta Agronomica Sinica
影响因子：
--
作者：
L. Hong;Liu Tai
通讯作者：
L. Hong;Liu Tai
影响因子：
5.1
作者：
Karrer, EE;Beachy, RN;Holt, CA
通讯作者：
Holt, CA
影响因子：
3.3
作者：
Liu, Xinqiong;Lin, Fei;Pan, Qinghua
通讯作者：
Pan, Qinghua
影响因子：
3.3
作者：
Gill, Bikram S.;Appels, Rudi;Sasaki, Takuji
通讯作者：
Sasaki, Takuji