Transcriptome analysis of cyst formation in Rhodospirillum centenum reveals large global changes in expression during cyst development.
Transcriptome analysis of cyst formation in Rhodospirillum centenum reveals large global changes in expression during cyst development.
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DOI：
10.1186/s12864-015-1250-9
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发表时间：
2015-02-13
期刊：
影响因子：
4.4
通讯作者：
Bauer CE
中科院分区：
文献类型：
--
作者：
Dong Q;Bauer CE
百蕊杜鹃是革兰氏阴性固氮菌属进化枝的光合成员，其表现出复杂的发育生命周期，其特征在于形态上不同的细胞类型。在营养缺乏的时期，复制营养细胞分化成代谢休眠的包囊，这些包囊在恶劣的环境压力（如干燥）中生存下来。包囊化涉及一个多阶段的发育过程，包括细胞变圆、产生大的聚羟基丁酸酯（PHB）细胞内储存颗粒和分泌一种保护性胞外多糖涂层，该多糖涂层包裹休眠的包囊。为了研究囊肿发育的过程，我们对被诱导经历囊肿发育的细胞进行了RNA-seq研究。为了测定基因表达的时间变化，在发育期间的4、24、48、72、96小时提取RNA并进行深度序列分析。这些结果表明，812个基因在96小时的囊肿诱导期内表现出log 2 ≥ 1.5倍的表达变化，表明在囊肿发育期间基因表达的大的整体变化。显著的表达变化发生在参与细胞壁和脂质生物合成的许多基因、代谢酶和许多调节基因如组氨酸激酶和转录因子中。许多参与蛋白质合成和DNA复制的基因在包囊发育的后期也显著减少。先前通过遗传筛选鉴定为对包囊发育至关重要的基因也在包囊诱导期间表现出表达的变化。这项研究提供了第一个转录组的基因表达的全球变化，发生在发展过程中的革兰氏阴性菌的囊肿。本文的在线版本（doi：10.1186/s12864-015-1250-9）包含补充材料，可供授权用户使用。
Rhodospirillum centenum is a photosynthetic member of the Gram-negative Azospirillum clade members of which exhibit a complex developmental life-cycle featuring morphologically distinct cell types. Under periods of nutrient deprivation, replicative vegetative cells differentiate into metabolically dormant cysts that survive harsh environmental stresses such as desiccation. Encystment involves a multi-stage developmental process that includes the rounding of cells, production of large intracellular storage granules of poly-hydroxybutyrate (PHB) and the excretion of a protective exopolysaccharide coating that envelops dormant cysts. To study the process of cyst development, we performed RNA-seq studies on cells that were induced to undergo cyst development. To assay for temporal changes in gene expression, RNA was extracted at 4, 24, 48, 72, 96 hours during development and subjected to deep sequence analysis. These results show that 812 genes exhibit log2 ≥ 1.5-fold changes in expression over a 96 hour cyst induction period demonstrating large global changes in gene expression during cyst development. Notable changes in expression occurred in numerous genes involved in cell wall and lipid biosynthesis, metabolic enzymes, and numerous regulatory genes such as histidine kinases and transcription factors. Many genes involved in protein synthesis and DNA replication were also significantly reduced during late stages of cyst development. Genes previously identified by genetic screens as being critical for cyst development also exhibited changes of expression during cyst induction. This study provides the first transcriptome profile of global changes in gene expression that occur during development of cysts in a Gram-negative species. The online version of this article (doi:10.1186/s12864-015-1250-9) contains supplementary material, which is available to authorized users.
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影响因子：
11.3
作者：
Higgins D;Dworkin J
通讯作者：
Dworkin J
影响因子：
4.4
作者：
Fang, Gang;Passalacqua, Karla D.;Jacobs-Wagner, Christine
通讯作者：
Jacobs-Wagner, Christine
影响因子：
3.2
作者：
Berleman, JE;Hasselbring, BM;Bauer, CE
通讯作者：
Bauer, CE
影响因子：
3.2
作者：
FROST, GE;ROSENBERG, H
通讯作者：
ROSENBERG, H
影响因子：
11.4
作者：
GILSON, L;MAHANTY, HK;KOLTER, R
通讯作者：
KOLTER, R