Large-scale Sequencing of Meiosis-associated Genes from a cDNA Library of Lily Microsporocytes
Large-scale Sequencing of Meiosis-associated Genes from a cDNA Library of Lily Microsporocytes
复制标题
百合小孢子母细胞 cDNA 文库中减数分裂相关基因的大规模测序
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
K. Hiratsuka
中科院分区：
文献类型：
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作者：
K. Morohashi;H. Takase;Y. Hotta;K. Hiratsuka
百合、长花百合具有花药内造孢细胞间发育同步的优点，已被用于减数分裂的研究。为了鉴定在减数分裂过程中表达的基因，我们对长花乳杆菌减数分裂前期合子期小孢子细胞的mRNAs文库进行了随机测序。为了避免cdna克隆的冗余和有效地获得新的序列信息，我们开发了一种简单而通用的“自杂交”策略。从418个克隆中获得了单次DNA序列数据。其中171个克隆的氨基酸序列与数据库中登记的氨基酸序列有显著的相似性，占所选克隆的41%。其中包括先前发现的减数分裂相关基因的cdna序列。然而，59%的选定克隆与已知基因产物没有显著的同源性。
Lily, Lilium longiflorum has been used to study meiosis for advantages of the synchronous development among sporogenous cells within an anther. In order to identify genes expressed at meiosis, we carried out random sequencing of a cDNA library which represented the mRNAs from microsporocytes of L. longiflorum at the zygotene stage of meiotic prophase. To avoid redundancy of cDNA clones and to obtain novel sequence information efficiently, we exploited a simple and versatile “self-hybridization” strategy. Single-run DNA sequence data were obtained from 418 cDNA clones. Deduced amino acid sequences of 171 cDNA clones, which comprised 41% of the selected cDNA clones, showed significant similarity to amino acid sequences registered in the database. These included cDNA sequences for previously identified meiosis-associated genes. However, 59% of the selected clones showed no significant homology to known gene products.
影响因子：
16
作者：
Pittman, DL;Cobb, J;Schimenti, JC
通讯作者：
Schimenti, JC