Expression of GABAergic and glutamatergic phenotypic markers in hypothalamic proopiomelanocortin neurons.
Expression of GABAergic and glutamatergic phenotypic markers in hypothalamic proopiomelanocortin neurons.
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DOI：
10.1002/cne.23127
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发表时间：
2012-12-01
影响因子：
2.5
通讯作者：
Hentges, Shane T.
中科院分区：
文献类型：
--
作者：
Jarvie, Brooke C.;Hentges, Shane T.
下丘脑前阿黑皮素（POMC）神经元传统上被定义为肽递质，这是重要的调节能量平衡和奖励。最近的工作表明，POMC神经元也可以释放氨基酸递质γ-氨基丁酸（GABA）和谷氨酸，尽管研究POMC神经元的GABA能和谷氨酸能群体一直受到难以可靠地鉴定氨基酸（AA）递质表型的阻碍。在本研究中，荧光原位杂交和免疫组织化学被用来确定POMC神经元和检测的存在下的转运体负责包装或GABA（囊泡GABA转运体[vGAT]）或谷氨酸（囊泡谷氨酸转运体[vGLUT]）到囊泡，以及负责GABA合成，谷氨酸脱羧酶（GAD）65和GAD 67的酶。大约7%的POMC神经元表达vGlut 2，并且vGlut 2阳性POMC细胞的最高百分比位于吻侧弓状核。尽管有从POMC神经元释放GABA的报道，但在POMC神经元中未检测到vGat，尽管Gad 65和Gad 67存在于约40%的POMC神经元中。大约一半的表达vGlut 2的POMC细胞也表达Gad 65。通过使用原位杂交技术，而不是在vGat或Gad 67启动子的控制下的荧光团的转基因表达，更好地检测神经递质表型的标志物。现在清楚的是，AA表型的标记物的表达提供了一种有用的手段来识别不同的POMC神经元亚群。此外，所描述的方法将是有用的，以探讨AA表型的可塑性是POMC神经元功能的重要方面的可能性。
Hypothalamic proopiomelanocortin (POMC) neurons have traditionally been defined by their peptide transmitters, which are important regulators of energy balance and reward. Recent work shows that POMC neurons can also release the amino acid transmitters γ-aminobutyric acid (GABA) and glutamate, although studying GABAergic and glutamatergic populations of POMC neurons has been hindered by the difficulty in reliably identifying amino acid (AA) transmitter phenotypes. In the present study, fluorescent in situ hybridization and immunohistochemistry were used to identify POMC neurons and to detect the presence of mRNA for the transporters responsible for packaging either GABA (vesicular GABA transporter [vGAT]) or glutamate (vesicular glutamate transporter [vGLUT]) into vesicles, as well as the enzymes responsible for GABA synthesis, glutamic acid decarboxylase (GAD)65 and GAD67. Approximately 7% of POMC neurons expressed vGlut2 and the highest percentage of vGlut2-positive POMC cells were located in the rostral arcuate nucleus. Despite the reports of GABA release from POMC neurons, vGat was not detected in POMC neurons, although Gad65 and Gad67 were present in ~40% of POMC neurons. Approximately half of the vGlut2-expressing POMC cells also expressed Gad65. Markers of neurotransmitter phenotype were better detected by using in situ hybridization techniques rather than transgenic expression of fluorophores under the control of the vGat or Gad67 promoters. It is now clear that the expression of markers of AA phenotype provides a useful means to identify distinct subpopulations of POMC neurons. Additionally, the method described will be useful to explore the possibility that plasticity of AA phenotype is an important aspect of POMC neuron function.
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影响因子：
3.7
作者：
Boulland, Jean-Luc;Jenstad, Monica;Chaudhry, Farrukh A.
通讯作者：
Chaudhry, Farrukh A.
DOI：
10.1523/jneurosci.3770-09.2009
发表时间：
2009-10-28
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Hentges ST;Otero-Corchon V;Pennock RL;King CM;Low MJ
通讯作者：
Low MJ
影响因子：
2.5
作者：
ESCLAPEZ, M;TILLAKARATNE, NJK;HOUSER, CR
通讯作者：
HOUSER, CR
影响因子：
5.3
作者：
Gómez-Lira, G;Lamas, M;Gutiérrez, R
通讯作者：
Gutiérrez, R
影响因子：
4.7
作者：
Fattorini, Giorgia;Verderio, Claudia;Conti, Fiorenzo
通讯作者：
Conti, Fiorenzo