Botulinum toxin A inhibits salivary secretion of rabbit submandibular gland.
Botulinum toxin A inhibits salivary secretion of rabbit submandibular gland.
复制标题
A型肉毒毒素抑制兔颌下腺唾液分泌
DOI：
10.1038/ijos.2013.82
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发表时间：
2013-12
影响因子：
14.9
通讯作者：
Yu, Guang-Yan
中科院分区：
文献类型：
--
作者：
Shan, Xiao-Feng;Xu, Hui;Cai, Zhi-Gang;Wu, Li-Ling;Yu, Guang-Yan
肉毒毒素A（BTXA）已在几个临床试验中用于治疗腺体分泌过多;然而，其对唾液腺分泌功能作用的确切机制尚未完全阐明。本研究旨在观察BTXA对家兔下颌下腺分泌功能的影响，并探讨其作用机制。在注射5单位BTXA后12周，我们发现下颌下腺的唾液流量显著减少，而唾液淀粉酶浓度增加。形态学分析显示腺泡细胞大小减少，细胞内分泌颗粒聚集形成大的卵圆形结构。注射BTXA后1、2和4周，M3受体和AQP 5 mRNA表达减少，AQP 5在根尖膜的分布减少。注射BTXA可诱导腺泡凋亡。这些结果表明，BTXA减少下颌下腺的液体分泌，并增加唾液中淀粉酶的浓度。M3受体和AQP 5表达减少、AQP 5转位抑制和细胞凋亡可能参与了BTXA减少下颌下腺液体分泌的作用。
Botulinum toxin A (BTXA) has been used in several clinical trials to treat excessive glandular secretion; however, the precise mechanism of its action on the secretory function of salivary gland has not been fully elucidated. In this study, we aimed to investigate the effect of BTXA on secretion of submandibular gland in rabbits and to identify its mechanism of action on the secretory function of salivary gland. At 12 weeks after injection with 5 units of BTXA, we found a significant decrease in the saliva flow from submandibular glands, while the salivary amylase concentration increased. Morphological analysis revealed reduction in the size of acinar cells with intracellular accumulation of secretory granules that coalesced to form a large ovoid structure. Expression of M3-muscarinic acetylcholine receptor (M3 receptor) and aquaporin-5 (AQP5) mRNA decreased after BTXA treatment, and distribution of AQP5 in the apical membrane was reduced at 1, 2 and 4 weeks after BTXA injection. Furthermore, BTXA injection was found to induce apoptosis of acini. These results indicate that BTXA decreases the fluid secretion of submandibular glands and increases the concentration of amylase in saliva. Decreased expression of M3 receptor and AQP5, inhibition of AQP5 translocation, and cell apoptosis might involve in BTXA-reduced fluid secretion of submandibular glands.
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影响因子：
3.8
作者：
Shi, L.;Cong, X.;Yu, G. Y.
通讯作者：
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影响因子：
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发表时间：
2008-01-01
影响因子：
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作者：
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通讯作者：
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