Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila.
Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila.
复制标题
DOI：
10.1016/j.celrep.2023.112723
复制
发表时间：
2023-07-25
期刊：
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
RNA颗粒是无膜浓缩物，在细胞内提供功能区室化。RNA颗粒形成的机制正在深入研究中。在这里，我们的特点的作用，基因和蛋白质在果蝇生殖颗粒的形成。超分辨率显微镜显示，胚芽颗粒的数量，大小和分布是精确控制的。令人惊讶的是，胚芽颗粒mRNA不是胚芽颗粒的成核或持久性所必需的，而是控制它们的大小和组成。利用RNAi筛选，我们确定RNA调节因子、解旋酶和线粒体蛋白调节胚芽颗粒的数量和大小，而内质网、核孔复合体和细胞骨架的蛋白控制它们的分布。因此，蛋白质驱动的果蝇胚芽颗粒的形成机制不同于其他RNA颗粒（如应激颗粒和P体）观察到的RNA依赖性缩合。Curnutte等人观察到，在果蝇中，胚粒蛋白而不是mRNA促进了Oskar胚粒的成核和稳定性。虽然RNA调节因子和线粒体蛋白控制着胚芽颗粒的数量和大小，但ER、核孔和细胞骨架的蛋白控制着它们的分布。
RNA granules are membraneless condensates that provide functional compartmentalization within cells. The mechanisms by which RNA granules form are under intense investigation. Here, we characterize the role of mRNAs and proteins in the formation of germ granules in Drosophila. Super-resolution microscopy reveals that the number, size, and distribution of germ granules is precisely controlled. Surprisingly, germ granule mRNAs are not required for the nucleation or the persistence of germ granules but instead control their size and composition. Using an RNAi screen, we determine that RNA regulators, helicases, and mitochondrial proteins regulate germ granule number and size, while the proteins of the endoplasmic reticulum, nuclear pore complex, and cytoskeleton control their distribution. Therefore, the protein-driven formation of Drosophila germ granules is mechanistically distinct from the RNA-dependent condensation observed for other RNA granules such as stress granules and P-bodies. Curnutte et al. observe that germ granule proteins rather than mRNAs promote the nucleation and stability of Oskar germ granules in Drosophila. While RNA regulators and mitochondrial proteins control the number and size of germ granules, the proteins of the ER, nuclear pores, and cytoskeleton control their distribution.
登录
查看更多内容
影响因子：
3.3
作者：
Durdevic, Zeljko;Ephrussi, Anne
通讯作者：
Ephrussi, Anne
影响因子：
7.7
作者：
Fuller, Gregory G.;Han, Ting;Kim, John K.
通讯作者：
Kim, John K.
DOI：
10.1083/jcb.200601156
发表时间：
2006-04-24
期刊：
The Journal of cell biology
影响因子：
--
作者：
Frescas D;Mavrakis M;Lorenz H;Delotto R;Lippincott-Schwartz J
通讯作者：
Lippincott-Schwartz J
影响因子：
4.5
作者：
Dold, Annabelle;Han, Hong;Lasko, Paul
通讯作者：
Lasko, Paul
影响因子：
64.8
作者：
Hachet, O;Ephrussi, A
通讯作者：
Ephrussi, A