The secretory membrane system in the Drosophila syncytial blastoderm embryo exists as functionally compartmentalized units around individual nuclei.
The secretory membrane system in the Drosophila syncytial blastoderm embryo exists as functionally compartmentalized units around individual nuclei.
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DOI：
10.1083/jcb.200601156
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发表时间：
2006-04-24
期刊：
影响因子：
--
通讯作者：
Lippincott-Schwartz J
中科院分区：
文献类型：
--
作者：
Frescas D;Mavrakis M;Lorenz H;Delotto R;Lippincott-Schwartz J
黑腹果蝇胚胎发生开始于合胞体内的13个核分裂周期。这会产生超过6，000个细胞核，在下一个分裂周期中，这些细胞核在称为细胞化的过程中被包裹在质膜中。在这项研究中，我们调查如何分泌膜系统成为平等地分配在成千上万的合胞体细胞核细胞化的准备。当细胞核到达皮层时，内质网（ER）和高尔基体被发现在细胞核之间分离，每个细胞核被一个与相邻细胞分离的ER/高尔基体膜系统包围。ER和高尔基体的核相关单位跨越合胞胚盘产生分泌产物，这些产物以空间受限的方式跨越胚胎被递送到质膜。这发生在细胞核之间的质膜边界的情况下，并依赖于中心体衍生的微管。在合胞胚盘中，分泌膜的出现可能确保分泌细胞器在细胞化时在细胞核之间被相等地分配，并且可能在早期胚胎内建立局部基因和蛋白质表达模式中发挥重要作用。
Drosophila melanogaster embryogenesis begins with 13 nuclear division cycles within a syncytium. This produces >6,000 nuclei that, during the next division cycle, become encased in plasma membrane in the process known as cellularization. In this study, we investigate how the secretory membrane system becomes equally apportioned among the thousands of syncytial nuclei in preparation for cellularization. Upon nuclear arrival at the cortex, the endoplasmic reticulum (ER) and Golgi were found to segregate among nuclei, with each nucleus becoming surrounded by a single ER/Golgi membrane system separate from adjacent ones. The nuclear-associated units of ER and Golgi across the syncytial blastoderm produced secretory products that were delivered to the plasma membrane in a spatially restricted fashion across the embryo. This occurred in the absence of plasma membrane boundaries between nuclei and was dependent on centrosome-derived microtubules. The emergence of secretory membranes that compartmentalized around individual nuclei in the syncytial blastoderm is likely to ensure that secretory organelles are equivalently partitioned among nuclei at cellularization and could play an important role in the establishment of localized gene and protein expression patterns within the early embryo.
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DOI：
10.1083/jcb.102.4.1494
发表时间：
1986-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Karr TL;Alberts BM
通讯作者：
Alberts BM
影响因子：
64.5
作者：
EDGAR, BA;SCHUBIGER, G
通讯作者：
SCHUBIGER, G
影响因子：
7.8
作者：
Miles, S;McManus, H;Storrie, B
通讯作者：
Storrie, B
影响因子：
3.3
作者：
Herpers, B;Rabouille, C
通讯作者：
Rabouille, C
影响因子：
4
作者：
Babu, P;Bryan, JD;Robinson, LC
通讯作者：
Robinson, LC