Bifidobacterium adolescentis Supplementation Ameliorates Parenteral Nutrition-Induced Liver Injury in Infant Rabbits
Bifidobacterium adolescentis Supplementation Ameliorates Parenteral Nutrition-Induced Liver Injury in Infant Rabbits
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补充青春双歧杆菌可改善幼兔肠外营养引起的肝损伤
DOI：
10.1007/s10620-009-1101-0
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发表时间：
2010-01
影响因子：
3.1
通讯作者：
Wu, Jiang
中科院分区：
文献类型：
--
作者：
Wang, Xiang;Cai, Wei;Hong, Li;Tang, Qingya;Wu, Jiang
背景：肠外营养（PN）引起的肝损伤与肠道萎缩有关，益生菌已被证明能够稳定肠道微生态系统并提供保护，防止细菌从肠道转移到肝脏。因此，我们推测肠内补充双歧杆菌可以减轻PN相关的肝损伤。方法：将3周龄新西兰兔分为三组：对照组、PN和PN+Bif组（PN加肠内喂养每天0.5×10(8)青春双歧杆菌）。 10天后，检测血清肝酶和内毒素水平，并进行肝脏和回肠的组织学检查。培养血液和来自肠系膜淋巴结、肺和脾的组织匀浆样本以检测细菌易位。通过糖吸收试验测定肠道通透性。结果：PN+Bif组血清总胆红素和胆汁酸水平较低，回肠和肝脏组织学明显改善（与PN组相比）。 PN+Bif组细菌移位率（15.6%）、血清内毒素水平（0.11±0.03 EU/ml）、乳果糖/甘露醇比值（0.02±0.004）均明显低于PN组（分别为77.5%、0.60±0.09 EU/ml、0.038±0.008），与对照组相似。对照组 （分别为 2.8%、0.09 ± 0.03 EU/ml 和 0.019 ± 0.005）。 结论：肠内补充益生菌可以降低肠道通透性、细菌移位和内毒素血症，从而减轻幼兔 PN 相关的肠道和肝脏损伤。
Background:Parenteral nutrition (PN)-induced liver injury is associated with gut atrophy, and probiotics have demonstrated the ability to stabilize the intestinal microecosystem and offer protection against bacterial translocation from the gut to the liver. Therefore, we hypothesized that enteral Bifidobacterium supplements could alleviate PN-associated liver injury.Methods:Three-week-old New Zealand rabbits were divided into three groups: control, PN, and PN + Bif group (PN plus enteral feeding 0.5 × 10(8) Bifidobacterium adolescentis per day). After 10 days, serum levels of liver enzyme and endotoxin were measured, and histology of liver and ileum were performed. Blood and homogenized samples of tissue from the mesenteric lymph nodes, lung, and spleen were cultured for detecting bacteria translocation. Intestinal permeability was determined by sugar absorption test.Results:Serum levels of total bilirubin and bile acid were found to be lower in the PN + Bif group, with considerably improved ileum and liver histology (vs. the PN group). The bacterial translocation rate (15.6%), serum endotoxin level (0.11 ± 0.03 EU/ml), and lactulose/mannitol ratio (0.02 ± 0.004) in the PN + Bif group were obviously lower than those of PN group (77.5%, 0.60 ± 0.09 EU/ml, and 0.038 ± 0.008, respectively) and similar to those of the control group (2.8%, 0.09 ± 0.03 EU/ml, and 0.019 ± 0.005, respectively).Conclusions:Enteral probiotic supplementation could reduce gut permeability, bacterial translocation and endotoxemia, and thus attenuate PN-associated gut and liver injuries in infant rabbits.
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DOI：
10.1093/ajcn/71.6.1603
发表时间：
2000-06
期刊：
The American journal of clinical nutrition
影响因子：
--
作者：
D. Burrin;B. Stoll;R. Jiang;X. Chang;B. Hartmann;J. Holst;G. Greeley;P. Reeds
通讯作者：
D. Burrin;B. Stoll;R. Jiang;X. Chang;B. Hartmann;J. Holst;G. Greeley;P. Reeds
影响因子：
3.8
作者：
J. Alverdy;E. Aoys;G. Moss
通讯作者：
J. Alverdy;E. Aoys;G. Moss
影响因子：
13.5
作者：
Adawi, D;Kasravi, FB;Jeppsson, B
通讯作者：
Jeppsson, B
影响因子：
3
作者：
H. Nakasaki;T. Mitomi;T. Tajima;N. Ohnishi;Koichi Fujii
通讯作者：
H. Nakasaki;T. Mitomi;T. Tajima;N. Ohnishi;Koichi Fujii
影响因子：
3.4
作者：
PAPPO, I;BERCOVIER, H;FREUND, HR
通讯作者：
FREUND, HR