Comparative Genomics of Facultative Bacterial Symbionts Isolated from European Orius Species Reveals an Ancestral Symbiotic Association.
Comparative Genomics of Facultative Bacterial Symbionts Isolated from European Orius Species Reveals an Ancestral Symbiotic Association.
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DOI：
10.3389/fmicb.2017.01969
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发表时间：
2017
影响因子：
5.2
通讯作者：
Del Sol R
中科院分区：
文献类型：
--
作者：
Chen X;Hitchings MD;Mendoza JE;Balanza V;Facey PD;Dyson PJ;Bielza P;Del Sol R
农业害虫防治采用多种策略，其中捕食性昆虫的使用稳步增加。在病虫害控制中广泛使用几个属的种，特别是在地中海欧洲。商业大规模饲养掠食性昆虫成本高昂，研究工作集中在饮食控制和选择性育种上，以降低成本和提高效率。微生物共生体的特征和对猪的适应性、行为和对人类健康的潜在影响的贡献一直被忽视。本文首次在基因组序列水平上描述了来自不同地理位置的五种猪（O. laevigatus， O. niger， O. pallidicornis， O. majusculus和O. albidipennis）的优势可培养兼性共生细菌。两种类型的共生体被广泛归类为沙雷氏菌属和白杆菌属的成员，而第三种构成了Erwiniaceae中的一个新属。这些共生体被发现在所有测试的昆虫标本中定植，这证明了这些细菌与Orius属之间的祖先共生关系。沙雷氏菌分离株的泛基因组分析提供了将Tai4亚家族的VI型分泌系统效应免疫蛋白与共生生活方式联系起来的线索。
Pest control in agriculture employs diverse strategies, among which the use of predatory insects has steadily increased. The use of several species within the genus Orius in pest control is widely spread, particularly in Mediterranean Europe. Commercial mass rearing of predatory insects is costly, and research efforts have concentrated on diet manipulation and selective breeding to reduce costs and improve efficacy. The characterisation and contribution of microbial symbionts to Orius sp. fitness, behaviour, and potential impact on human health has been neglected. This paper provides the first genome sequence level description of the predominant culturable facultative bacterial symbionts associated with five Orius species (O. laevigatus, O. niger, O. pallidicornis, O. majusculus, and O. albidipennis) from several geographical locations. Two types of symbionts were broadly classified as members of the genera Serratia and Leucobacter, while a third constitutes a new genus within the Erwiniaceae. These symbionts were found to colonise all the insect specimens tested, which evidenced an ancestral symbiotic association between these bacteria and the genus Orius. Pangenome analyses of the Serratia sp. isolates offered clues linking Type VI secretion system effector–immunity proteins from the Tai4 sub-family to the symbiotic lifestyle.
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影响因子：
3.3
作者：
Facey PD;Méric G;Hitchings MD;Pachebat JA;Hegarty MJ;Chen X;Morgan LV;Hoeppner JE;Whitten MM;Kirk WD;Dyson PJ;Sheppard SK;Del Sol R
通讯作者：
Del Sol R
DOI：
10.1099/ijs.0.63674-0
发表时间：
2005-09-01
影响因子：
2.8
作者：
Bhadra, B;Roy, P;Chakraborty, R
通讯作者：
Chakraborty, R
影响因子：
3
作者：
Meier-Kolthoff JP;Auch AF;Klenk HP;Göker M
通讯作者：
Göker M
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
影响因子：
3.6
作者：
English G;Trunk K;Rao VA;Srikannathasan V;Hunter WN;Coulthurst SJ
通讯作者：
Coulthurst SJ