Glycyrrhiza uralensis flavonoids present in anti-asthma formula, ASHMI™, inhibit memory Th2 responses in vitro and in vivo.
Glycyrrhiza uralensis flavonoids present in anti-asthma formula, ASHMI™, inhibit memory Th2 responses in vitro and in vivo.
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DOI：
10.1002/ptr.4862
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发表时间：
2013-09
影响因子：
7.2
通讯作者：
Li, Xiu-Min
中科院分区：
文献类型：
--
作者：
Yang, Nan;Patil, Sangita;Jian Zhuge;Wen, Ming-Chun;Bolleddula, Jayaprakasam;Doddaga, Srinivasulu;Goldfarb, Joseph;Sampson, Hugh A.;Li, Xiu-Min
过敏性哮喘与Th 2介导的炎症相关。从甘草中分离出几种黄酮类化合物，甘草是抗哮喘草药干预的草药之一，ASHMI。本研究的目的是确定甘草黄酮类化合物是否具有抑制记忆性Th 2应答的体外作用，以及抑制抗原诱导的Th 2炎症的体内作用。通过测定Th 2细胞因子的产生来确定三种甘草黄酮对效应记忆Th 2细胞D10.G4.1（D10细胞）的影响。异甘草素、7，4 ′-二羟基黄酮（7，4 ′-DHF）和甘草素以剂量依赖性方式显著抑制IL-4和IL-5的产生，其中7，4 ′-DHF最有效。还评价了其对D10细胞增殖、加塔-3表达和IL-4 mRNA表达的影响，其被抑制，而没有细胞活力的损失。在过敏性哮喘的小鼠模型中，用7，4 '-DHF长期治疗不仅显著降低嗜酸性粒细胞性肺部炎症、血清IgE水平、IL-4和IL-13水平，而且增加肺细胞培养物中响应于抗原刺激的IFN-γ产生。
Allergic asthma is associated with Th2-mediated inflammation. Several flavonoids were isolated from Glycyrrhiza uralensis, one of the herbs in the anti-asthma herbal medicine intervention, ASHMI. The aim of this investigation was to determine whether Glycyrrhiza uralensis flavonoids have inhibitory effects on memory Th2 responses in vitro, and antigen induced Th2 inflammation in vivo. The effects of three Glycyrrhiza uralensis flavonoids on effector memory Th2 cells, D10.G4.1 (D10 cells), were determined by measuring Th2 cytokine production. Isoliquiritigenin, 7, 4’-dihydroxyflavone (7, 4’-DHF) and liquiritigenin significantly suppressed IL-4 and IL-5 production in a dose dependent manner, 7, 4’-DHF being most potent. It was also evaluated for effects on D10 cell proliferation, GATA-3 expression and IL-4 mRNA expression, which were suppressed, with no loss of cell viability. Chronic treatment with 7, 4’-DHF in a murine model of allergic asthma not only significantly reduced eosinophilic pulmonary inflammation, serum IgE levels, IL-4 and IL-13 levels, but also increased IFN-γ production in lung cell cultures in response to antigen stimulation.
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影响因子：
8
作者：
Akinbami, Lara J.;Moorman, Jeanne E.;Sondik, Edward J.
通讯作者：
Sondik, Edward J.
影响因子：
5.4
作者：
PENE, J;ROUSSET, F;DEVRIES, JE
通讯作者：
DEVRIES, JE
DOI：
10.4049/jimmunol.0902185
发表时间：
2010-02-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Finkelman FD;Hogan SP;Hershey GK;Rothenberg ME;Wills-Karp M
通讯作者：
Wills-Karp M
影响因子：
64.8
作者：
Sallusto, F;Lenig, D;Lanzavecchia, A
通讯作者：
Lanzavecchia, A
影响因子：
5.7
作者：
Sheth, A;Reddymasu, S;Jackson, R
通讯作者：
Jackson, R