Inorganic Nitrogen Transport and Assimilation in Pea (Pisum sativum).
Inorganic Nitrogen Transport and Assimilation in Pea (Pisum sativum).
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DOI：
10.3390/genes13010158
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发表时间：
2022-01-17
期刊：
影响因子：
3.5
通讯作者：
Miller AJ
中科院分区：
文献类型：
--
作者：
Gu B;Chen Y;Xie F;Murray JD;Miller AJ
几个豆科植物物种的基因组序列现已可供比较氮（N）运输库存与非豆科植物物种。对豌豆中无机氮转运蛋白和感生同化家族的基因进行了调查，发现与其他类型的植物中编码初级氮同化酶的基因数量相似。有趣的是，我们发现豌豆和蒺藜苜蓿具有较少的NRT 2硝酸盐转运蛋白家族成员。我们认为，这种差异可能是由于减少依赖土壤硝酸盐的收购，豆科植物有能力获得氮与固氮共生关系。与M比较truncatula，表明豌豆中的三个NRT 2中只有一个可能是功能性的，可能表明在根瘤形成和固氮开始之前氮吸收较少。豌豆种子很大，含有大量的富含氮的储存蛋白，提供了一个储备，有助于幼苗的建立，这也可以解释为什么需要较少的高亲和力硝酸盐转运蛋白。液泡中硝酸盐积累的能力是同化的另一个组成部分，因为它可以提供一个储存库，在土壤氮耗尽时为植物提供营养。比较已发表的豌豆组织硝酸盐浓度与其他植物，我们发现，有较少的硝酸盐积累，即使在非pellulated植物，这表明一个较低的能力为液泡存储。有机氮在韧皮部中的长距离运输形式在豆科植物中是专门的，其中运输的有机氮分子的量增加，如豌豆中的酰脲、尿囊素、天冬酰胺和酰胺。我们认为，在一般情况下，较低的组织和韧皮部的硝酸盐水平相比，非豆科植物也可能会导致对高亲和力的硝酸盐转运蛋白的需求减少。讨论了豌豆氮转运蛋白和同化酶的分布模式，并与非豆科植物进行了比较，旨在确定未来的育种目标。
The genome sequences of several legume species are now available allowing the comparison of the nitrogen (N) transporter inventories with non-legume species. A survey of the genes encoding inorganic N transporters and the sensing and assimilatory families in pea, revealed similar numbers of genes encoding the primary N assimilatory enzymes to those in other types of plants. Interestingly, we find that pea and Medicago truncatula have fewer members of the NRT2 nitrate transporter family. We suggest that this difference may result from a decreased dependency on soil nitrate acquisition, as legumes have the capacity to derive N from a symbiotic relationship with diazotrophs. Comparison with M. truncatula, indicates that only one of three NRT2s in pea is likely to be functional, possibly indicating less N uptake before nodule formation and N-fixation starts. Pea seeds are large, containing generous amounts of N-rich storage proteins providing a reserve that helps seedling establishment and this may also explain why fewer high affinity nitrate transporters are required. The capacity for nitrate accumulation in the vacuole is another component of assimilation, as it can provide a storage reservoir that supplies the plant when soil N is depleted. Comparing published pea tissue nitrate concentrations with other plants, we find that there is less accumulation of nitrate, even in non-nodulated plants, and that suggests a lower capacity for vacuolar storage. The long-distance transported form of organic N in the phloem is known to be specialized in legumes, with increased amounts of organic N molecules transported, like ureides, allantoin, asparagine and amides in pea. We suggest that, in general, the lower tissue and phloem nitrate levels compared with non-legumes may also result in less requirement for high affinity nitrate transporters. The pattern of N transporter and assimilatory enzyme distribution in pea is discussed and compared with non-legumes with the aim of identifying future breeding targets.
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影响因子：
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