Methods for the Comprehensive in vivo Analysis of Energy Flux, Fluid Homeostasis, Blood Pressure, and Ventilatory Function in Rodents.
Methods for the Comprehensive in vivo Analysis of Energy Flux, Fluid Homeostasis, Blood Pressure, and Ventilatory Function in Rodents.
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DOI：
10.3389/fphys.2022.855054
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发表时间：
2022
影响因子：
4
通讯作者：
Grobe JL
中科院分区：
文献类型：
--
作者：
Reho JJ;Nakagawa P;Mouradian GC Jr;Grobe CC;Saravia FL;Burnett CML;Kwitek AE;Kirby JR;Segar JL;Hodges MR;Sigmund CD;Grobe JL
在美国，心血管疾病是导致死亡的主要原因，而肥胖等代谢性疾病是改善心血管健康的主要障碍。啮齿类动物（小鼠和大鼠）模型被广泛用于模拟心脏代谢疾病，因此，人们对开发具有足够高分辨率的准确和精确的方法来解剖这些小生物控制心脏代谢生理学的机制越来越感兴趣。此外，开发配备高通量设备配置并配备专业内容专家的集中式核心设施具有很大的实用性，以指导研究人员并确保实验努力的严谨性和可重复性。在这里，我们概述了一系列专门的设备和方法，这些设备和方法是在综合啮齿动物代谢表型核心（CRMPC）和我们在威斯康星医学院（MCW）生理学，儿科，微生物学和免疫学以及生物医学工程系的合作实验室中使用的，用于小鼠和大鼠心脏代谢功能的详细机制解剖。我们重点介绍了分析身体组成和液体区隔、电解质积累和通量、能量积累和通量、身体活动、摄食行为、呼吸功能、血压、心率、自主神经功能以及肠道微生物群评估和控制的方法。此外，我们还讨论了这些方法在啮齿动物模型中使用的优点和缺点，以及使用这些方法进行实验设计的考虑。
Cardiovascular disease represents the leading cause of death in the United States, and metabolic diseases such as obesity represent the primary impediment to improving cardiovascular health. Rodent (mouse and rat) models are widely used to model cardiometabolic disease, and as a result, there is increasing interest in the development of accurate and precise methodologies with sufficiently high resolution to dissect mechanisms controlling cardiometabolic physiology in these small organisms. Further, there is great utility in the development of centralized core facilities furnished with high-throughput equipment configurations and staffed with professional content experts to guide investigators and ensure the rigor and reproducibility of experimental endeavors. Here, we outline the array of specialized equipment and approaches that are employed within the Comprehensive Rodent Metabolic Phenotyping Core (CRMPC) and our collaborating laboratories within the Departments of Physiology, Pediatrics, Microbiology & Immunology, and Biomedical Engineering at the Medical College of Wisconsin (MCW), for the detailed mechanistic dissection of cardiometabolic function in mice and rats. We highlight selected methods for the analysis of body composition and fluid compartmentalization, electrolyte accumulation and flux, energy accumulation and flux, physical activity, ingestive behaviors, ventilatory function, blood pressure, heart rate, autonomic function, and assessment and manipulation of the gut microbiota. Further, we include discussion of the advantages and disadvantages of these approaches for their use with rodent models, and considerations for experimental designs using these methods.
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影响因子：
11.1
作者：
Bahr SM;Weidemann BJ;Castro AN;Walsh JW;deLeon O;Burnett CM;Pearson NA;Murry DJ;Grobe JL;Kirby JR
通讯作者：
Kirby JR
影响因子：
6.3
作者：
Abais-Battad, Justine M.;Saravia, Fatima L.;Lund, Hayley;Dasinger, John Henry;Fehrenbach, Daniel J.;Alsheikh, Ammar J.;Zemaj, Jeylan;Kirby, John R.;Mattson, David L.
通讯作者：
Mattson, David L.
影响因子：
37.8
作者：
Angell SY;McConnell MV;Anderson CAM;Bibbins-Domingo K;Boyle DS;Capewell S;Ezzati M;de Ferranti S;Gaskin DJ;Goetzel RZ;Huffman MD;Jones M;Khan YM;Kim S;Kumanyika SK;McCray AT;Merritt RK;Milstein B;Mozaffarian D;Norris T;Roth GA;Sacco RL;Saucedo JF;Shay CM;Siedzik D;Saha S;Warner JJ
通讯作者：
Warner JJ
DOI：
10.1093/oso/9780198830399.001.0001
发表时间：
2019-01-01
期刊：
MEASURING METABOLIC RATES: A MANUAL FOR SCIENTISTS, 2ND EDITION
影响因子：
--
作者：
Lighton, J. R. B.
通讯作者：
Lighton, J. R. B.
影响因子：
5.5
作者：
Mouradian, Gary C., Jr.;Forster, Hubert V.;Hodges, Matthew R.
通讯作者：
Hodges, Matthew R.