Acute treatment with 17β‐estradiol attenuates astrocyte–astrocyte and astrocyte–neuron communication
Acute treatment with 17β‐estradiol attenuates astrocyte–astrocyte and astrocyte–neuron communication
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17β-雌二醇急性治疗会减弱星形胶质细胞-星形胶质细胞和星形胶质细胞-神经元的通讯
作者：
S. Rao;S. Sikdar
星形胶质细胞现在被认为是大脑中的动态信号元件。神经元和星形胶质细胞之间的双向通信涉及星形胶质细胞对神经元输入的整合以及调节神经元兴奋性和突触传递的胶质递质的释放。卵巢类固醇激素17β-雌二醇除了对神经元电活动的快速作用外，还可通过膜相关雌激素受体快速改变星形胶质细胞内钙浓度（[Ca 2 +]i）。使用钙成像和电生理技术，我们研究了急性治疗与雌二醇对星形胶质细胞和星形胶质细胞神经元通信混合海马文化的功能后果。星形胶质细胞的机械刺激诱发了[Ca 2 +]i在刺激的星形胶质细胞中的上升，其作为[Ca 2 +]i波传播到周围的星形胶质细胞。雌二醇急性治疗后，[Ca 2 +]i升高的幅度在星形胶质细胞周围的刺激星形胶质细胞减弱。此外，雌二醇抑制了代谢型谷氨酸受体激动剂反式-（±）-1-氨基-1，3-环戊烷二羧酸引起的单个星形胶质细胞[Ca 2 +]i升高。星形胶质细胞的机械刺激诱导[Ca 2 +]i升高和邻近神经元的电生理反应。肾上腺素能迅速减弱星形胶质细胞诱发的谷氨酸介导的[Ca 2 +]i升高和神经元缓慢内向电流。此外，雌二醇存在时，星形胶质细胞诱导的自发突触后电流频率增加的发生率降低。雌二醇的作用是立体特异性的，洗脱后可逆。这些发现可能表明星形胶质细胞对神经元兴奋性和突触传递的调节对快速雌二醇介导的激素控制敏感。© 2007 Wiley利斯公司
Astrocytes are now recognized as dynamic signaling elements in the brain. Bidirectional communication between neurons and astrocytes involves integration of neuronal inputs by astrocytes and release of gliotransmitters that modulate neuronal excitability and synaptic transmission. The ovarian steroid hormone, 17β‐estradiol, in addition to its rapid actions on neuronal electrical activity can rapidly alter astrocyte intracellular calcium concentration ([Ca2+]i) through a membrane‐associated estrogen receptor. Using calcium imaging and electrophysiological techniques, we investigated the functional consequences of acute treatment with estradiol on astrocyte–astrocyte and astrocyte–neuron communication in mixed hippocampal cultures. Mechanical stimulation of an astrocyte evoked a [Ca2+]i rise in the stimulated astrocyte, which propagated to the surrounding astrocytes as a [Ca2+]i wave. Following acute treatment with estradiol, the amplitude of the [Ca2+]i elevation in astrocytes around the stimulated astrocyte was attenuated. Further, estradiol inhibited the [Ca2+]i rise in individual astrocytes in response to the metabotropic glutamate receptor agonist, trans‐(±)‐1‐amino‐1,3‐cyclopentanedicarboxylic acid. Mechanical stimulation of astrocytes induced [Ca2+]i elevations and electrophysiological responses in adjacent neurons. Estradiol rapidly attenuated the astrocyte‐evoked glutamate‐mediated [Ca2+]i rise and slow inward current in neurons. Also, the incidence of astrocyte‐induced increase in spontaneous postsynaptic current frequency was reduced in the presence of estradiol. The effects of estradiol were stereo‐specific and reversible following washout. These findings may indicate that the regulation of neuronal excitability and synaptic transmission by astrocytes is sensitive to rapid estradiol‐mediated hormonal control. © 2007 Wiley‐Liss, Inc.
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DOI：
10.1073/pnas.2630225100
发表时间：
2004-01-20
影响因子：
11.1
作者：
Hojo, Y;Hattori, T;Kawato, S
通讯作者：
Kawato, S
磷脂酶 C 活性而非兰尼碱受体在细胞间钙波的启动和传播中发挥作用。
影响因子：
4
作者：
Hansen,M;Boitano,S;Dirksen,ER;Sanderson,MJ
通讯作者：
Sanderson,MJ
DOI：
10.1152/ajpendo.2000.279.4.e823
发表时间：
2000-10
期刊：
American journal of physiology. Endocrinology and metabolism
影响因子：
--
作者：
M. Ryan;K. Gross;G. Hajduczok
通讯作者：
M. Ryan;K. Gross;G. Hajduczok
超微结构证据表明海马 α 雌激素受体位于核外位点。
DOI：
--
发表时间：
2001
期刊：
The Journal of comparative neurology.
影响因子：
--
作者：
Milner,TA;McEwen,BS;Hayashi,S;Li,CJ;Reagan,LP;Alves,SE
通讯作者：
Alves,SE
影响因子：
56.9
作者：
CORNELLBELL, AH;FINKBEINER, SM;SMITH, SJ
通讯作者：
SMITH, SJ