Protocol for Microbiome Transplantation in Suckling Mice during Vibrio cholerae Infection to Study Commensal-Pathogen Interactions.
Protocol for Microbiome Transplantation in Suckling Mice during Vibrio cholerae Infection to Study Commensal-Pathogen Interactions.
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DOI：
10.1016/j.xpro.2020.100200
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发表时间：
2020-12-18
期刊：
影响因子：
--
通讯作者：
Hsiao A
中科院分区：
文献类型：
--
作者：
Alavi S;Hsiao A
肠道微生物组在排除病原体方面起着重要作用，因此，感染结果。微生物组-病原体相互作用研究由于缺乏易处理的动物模型以及动物模型与人类微生物组的差异而变得复杂。我们已经适应了人类病原体霍乱弧菌感染的乳鼠模型，以清除鼠微生物，并在感染过程中建立人类相关的肠道微生物。我们的方法可以很容易地检查不同的人类微生物群落对肠道致病的贡献。有关本方案使用和执行的完整详细信息，请参阅。测试人类微生物组和霍乱弧菌相互作用的方案小鼠肠道菌群的消耗允许用人类肠道细菌替代制备细菌混合物和乳鼠灌胃的详细程序肠道微生物组在排除病原体和感染结果中起着重要作用。微生物组-病原体相互作用研究由于缺乏易处理的动物模型以及动物模型与人类微生物组的差异而变得复杂。我们已经适应了人类病原体霍乱弧菌感染的乳鼠模型，以清除鼠微生物，并在感染过程中建立人类相关的肠道微生物。我们的方法可以很容易地检查不同的人类微生物群落对肠道致病的贡献。
The gut microbiome plays an important role in the exclusion of pathogens and, thus, infection outcomes. Microbiome-pathogen interaction studies are complicated by a lack of tractable animal models and differences in animal model versus human microbiomes. We have adapted the suckling mouse model of infection of the human pathogen Vibrio cholerae to clear murine microbes and establish human-associated gut microbes during infection. Our method allows for the easy examination of the contribution of different human microbial communities to enteropathogenesis. For complete details on the use and execution of this protocol, please refer to. A protocol for testing interactions of human microbiomes and Vibrio cholerae Depletion of murine commensals allows for replacement with human gut bacteria Detailed procedures for preparation of bacterial mixtures and gavage of suckling mice The gut microbiome plays an important role in the exclusion of pathogens and, thus, infection outcomes. Microbiome-pathogen interaction studies are complicated by a lack of tractable animal models and differences in animal model versus human microbiomes. We have adapted the suckling mouse model of infection of the human pathogen Vibrio cholerae to clear murine microbes and establish human-associated gut microbes during infection. Our method allows for the easy examination of the contribution of different human microbial communities to enteropathogenesis.
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影响因子：
3.7
作者：
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64.5
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DOI：
10.1073/pnas.0604650103
发表时间：
2006-09-26
影响因子：
11.1
作者：
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Zhu, Jun
影响因子：
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