Identification of brain isoforms of the rat calcitonin receptor.
Identification of brain isoforms of the rat calcitonin receptor.
复制标题
大鼠降钙素受体脑亚型的鉴定。
作者：
P. Sexton;S. Houssami;J. Hilton;L. M. O'Keeffe;R. J. Center;M. Gillespie;P. Darcy;D. Findlay
从大鼠下丘脑cDNA文库中克隆了两种降钙素受体亚型。克隆C1a和C1b分别指定了478和515个氨基酸的蛋白质。克隆是相同的，除了C1b序列编码的第二胞外结构域中的37个氨基酸的插入，这赋予改变配体识别。与C1a受体相比，表达的C1b受体对猪CT的亲和力相对于鲑鱼CT降低，对人CT的亲和力可忽略不计。克隆C1b mRNA主要在脑中表达，而C1a克隆的mRNA存在于脑和外周组织中。这两种受体都能够在功能上与腺苷酸环化酶偶联。因此，克隆C1b代表CT受体的一种新的脑同种型，其对CT类似物具有不同的亲和力，这是由改变的第二胞外结构域引起的。
Two rat calcitonin receptor isoforms have been identified by cDNA cloning from a hypothalamic library. The clones, C1a and C1b, specified proteins of 478 and 515 amino acids, respectively. The clones were identical, except that the C1b sequence encoded a 37-amino acid insert in the second extracellular domain, which conferred altered ligand recognition. Compared to the C1a receptor, expressed C1b receptors exhibited decreased affinity for porcine CT, relative to salmon CT, and negligible affinity for human CT. Clone C1b mRNA was predominately expressed in the brain, whereas mRNA for the C1a clone was present in both brain and peripheral tissues. Both receptors were able to couple functionally to adenylate cyclase. Thus, clone C1b represents a novel brain isoform of the CT receptor with different affinity for CT analogs resulting from an altered second extracellular domain.
影响因子：
56.9
作者：
CHAKRABORTY, M;CHATTERJEE, D;BARON, R
通讯作者：
BARON, R