A protocol for precise comparisons of small vessel disease lesions between ex vivo magnetic resonance imaging and histopathology.
A protocol for precise comparisons of small vessel disease lesions between ex vivo magnetic resonance imaging and histopathology.
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DOI：
10.1177/1747493018799962
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发表时间：
2019-04
期刊：
影响因子：
--
通讯作者：
Smith C
中科院分区：
文献类型：
--
作者：
Humphreys CA;Jansen MA;Muñoz Maniega S;González-Castro V;Pernet C;Deary IJ;Al-Shahi Salman R;Wardlaw JM;Smith C
神经影像学和临床研究已经确定了人类散发性脑小血管疾病，但其病理生理机制仍然知之甚少。为了开发有效的治疗和预防策略，我们必须在细胞水平上更好地了解小血管疾病的异质性和发展。神经影像学上所见的小血管病变具有特定的神经病理学相关性。标准组织学样本取自通常受小血管疾病影响的脑的关键区域，在具有从轻度到重度的一系列疾病的病例和对照中。将组织用福尔马林固定，使用7特斯拉磁共振成像扫描并进行组织学处理。组织切片被数字化，然后与相应的磁共振图像配准。评估小血管疾病负荷，并在离体成像和显微镜检查中独立地精确识别病变，然后进行比较。可以使用多个磁共振序列和靶向胶质血管单元的组织学方法来询问组织。主要结果是识别和定义与成像相比的小血管病变的细胞特征。次要结局与获得有关胶质血管单位蛋白质表达异常的信息有关，在我们的队列中定义小血管疾病严重程度的组以供未来分析，并开发可靠的，可重复的方案以进行准确的放射学-组织学病变比较，将来可应用于其他神经系统疾病。在小血管疾病中全面、精确的病理-放射学-临床相关性将为离体和体内正常和异常组织的磁共振成像结果的相关性和病理生理学提供更深入的了解。
Neuroimaging and clinical studies have defined human sporadic cerebral small vessel disease but the pathophysiology remains relatively poorly understood. To develop effective therapies and preventative strategies, we must better understand the heterogeneity and development of small vessel disease at a cellular level. Small vessel disease lesions as seen on neuroimaging have specific neuropathological correlates. Standard histological samples are taken from strategic areas of the brain typically affected by small vessel disease, in cases with a range of disease from mild to severe and controls. Tissue is formalin fixed, scanned using 7-tesla magnetic resonance imaging and processed for histology. Histological slides are digitalized then registered with the corresponding magnetic resonance image. Small vessel disease burden is assessed and lesions are precisely identified on the ex vivo imaging and microscopy independently then compared. The tissue can be interrogated using multiple magnetic resonance sequences and histological methods targeting the gliovascular unit. The primary outcome is identifying and defining the cellular characteristics of small vessel disease lesions compared to imaging. Secondary outcomes are related to obtaining information about abnormalities of protein expression in the gliovascular unit, defining groups of small vessel disease severity in our cohorts for future analysis and developing a reliable, reproducible protocol for accurate radiological–histological lesion comparison, which can be applied to other neurological diseases in the future. Comprehensive, precise pathological–radiological–clinical correlations in small vessel disease will provide greater insight into associations and pathophysiology underlying magnetic resonance imaging findings in normal- and abnormal-appearing tissue, ex vivo and in vivo.
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DOI：
10.1093/brain/awv328
发表时间：
2016-01
期刊：
Brain : a journal of neurology
影响因子：
--
作者：
Chen A;Akinyemi RO;Hase Y;Firbank MJ;Ndung'u MN;Foster V;Craggs LJ;Washida K;Okamoto Y;Thomas AJ;Polvikoski TM;Allan LM;Oakley AE;O'Brien JT;Horsburgh K;Ihara M;Kalaria RN
通讯作者：
Kalaria RN
影响因子：
4.1
作者：
Deary IJ;Gow AJ;Taylor MD;Corley J;Brett C;Wilson V;Campbell H;Whalley LJ;Visscher PM;Porteous DJ;Starr JM
通讯作者：
Starr JM
影响因子：
--
作者：
Lin J;Wang D;Lan L;Fan Y
通讯作者：
Fan Y
影响因子：
5
作者：
Bailey EL;McBride MW;Beattie W;McClure JD;Graham D;Dominiczak AF;Sudlow CL;Smith C;Wardlaw JM
通讯作者：
Wardlaw JM
影响因子：
5
作者：
BRAFFMAN, BH;ZIMMERMAN, RA;SCHLAEPFER, WW
通讯作者：
SCHLAEPFER, WW