Comparison of three multiple allergen simultaneous tests: RIDA allergy screen, MAST optigen, and polycheck allergy.
Comparison of three multiple allergen simultaneous tests: RIDA allergy screen, MAST optigen, and polycheck allergy.
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DOI：
10.1155/2013/340513
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发表时间：
2013
影响因子：
--
通讯作者：
Song EY
中科院分区：
文献类型：
--
作者：
Han M;Shin S;Park H;Park KU;Park MH;Song EY
我们比较了3种多种过敏原同时检测（MAST）检测试剂盒的性能：RIDA过敏筛查（R-Biopharm，达姆施塔特，德国）、MAST Optigen过敏系统（Hitachi Chemical Diagnostics，山景，CA）和Polycheck过敏（Biocheck GmbH，Munster，德国）。对40份经RIDA过敏筛查检测为阳性的血清（20份用于食物，20份用于吸入剂组）进行MAST Optigen和Polycheck过敏试验。对于具有不一致结果的26份可用血清，进行了62次ImmunoCAP过敏原特异性IgE检测（Pharmacia Diagnostics，Uppsala，Sweden）。RIDA和MAST之间的一致性百分比（kappa值）分别为87.6%（0.59）和91.3%（0.60）; RIDA和Polycheck之间的一致性百分比分别为89.9%（0.55）和88.3%（0.46）; MAST和Polycheck之间的一致性百分比分别为86.8%（0.51）和90.6%（0.61）。与ImmunoCAP相比，RIDA吸入剂和食物组的一致性（kappa值）分别为51.7%（0.04）和33.3%（-0.38）; MAST为60.7%（0.27）和81.8%（0.59）; Polycheck为65.5%（0.26）和45.5%（0.07）。RIDA、MAST和Polycheck与ImmunoCAP阳性的符合率分别为45.7%、88.2%和28.6%，与ImmunoCAP阴性的符合率分别为37.0%、51.9%和88.9%。MAST Optigen与ImmunoCAP的一致性优于食品组中的其他检测。怀疑MAST Optigen的灵敏度更高，Polycheck Allergy的特异性更高。
We compared the performances of 3 Multiple Allergen Simultaneous Test (MAST) assays: RIDA Allergy Screen (R-Biopharm, Darmstadt, Germany), MAST Optigen allergy system (Hitachi Chemical Diagnostics, Mountain View, CA), and Polycheck Allergy (Biocheck GmbH, Munster, Germany). Forty sera that tested positive with the RIDA Allergy Screen (20 for food and 20 for inhalant panel) were subjected to MAST Optigen and Polycheck Allergy. For 26 available sera with discrepant results, 62 ImmunoCAP allergen-specific IgE tests (Pharmacia Diagnostics, Uppsala, Sweden) were performed. Percent agreements (kappa value) were 87.6% (0.59) and 91.3% (0.60) between RIDA and MAST; 89.9% (0.55) and 88.3% (0.46) between RIDA and Polycheck; and 86.8% (0.51) and 90.6% (0.61) between MAST and Polycheck. Compared with ImmunoCAP, agreements (kappa value) of inhalant and food panels were 51.7% (0.04) and 33.3% (−0.38) for RIDA; 60.7% (0.27) and 81.8% (0.59) for MAST; and 65.5% (0.26) and 45.5% (0.07) for Polycheck. The agreements between RIDA, MAST, and Polycheck and ImmunoCAP-positivity were 45.7%, 88.2%, and 28.6%, respectively, and the agreements for ImmunoCAP-negativity were 37.0%, 51.9%, and 88.9%. MAST Optigen showed better agreement with ImmunoCAP than other assays in the food panel. Better sensitivity of MAST Optigen and better specificity of Polycheck Allergy were suspected.
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影响因子：
12.4
作者：
Linneberg, A;Husemoen, LLN;Johansen, N
通讯作者：
Johansen, N
影响因子：
--
作者：
Jang, Woo Ri;Nahm, Chung Hyun;Kim, Jin Ju
通讯作者：
Kim, Jin Ju
影响因子：
1.9
作者：
LANDIS, JR;KOCH, GG
通讯作者：
KOCH, GG
影响因子：
5
作者：
Lee, Sungsil;Lim, Hwan Sub;Kim, Hyon Sur
通讯作者：
Kim, Hyon Sur
影响因子：
4.6
作者：
Williams, P.;Sewell, W. A. C.;Jolles, S.
通讯作者：
Jolles, S.