Increase in number of Weibel-Palade bodies and endothelin-1 release from endothelial cells in the cadmium-treated rat thoracic aorta
Increase in number of Weibel-Palade bodies and endothelin-1 release from endothelial cells in the cadmium-treated rat thoracic aorta
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镉处理的大鼠胸主动脉内皮细胞 Weibel-Palade 小体数量增加和内皮素 1 释放增加
DOI：
10.1007/bf00202203
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发表时间：
1996
期刊：
影响因子：
3.5
通讯作者：
M. Yoshizuka
中科院分区：
文献类型：
--
作者：
Y. Doi;T. Ozaka;H. Fukushige;S. Fujimoto;H. Furukawa;M. Yoshizuka
雄性大鼠腹腔注射硫酸镉（2.0mg/kg），连续3（Cd-3组）、6（Cd-6组）和8（Cd-8组）d。取血测定内皮素（ET-1），用抗ET-1血清对胸主动脉进行电镜和免疫电镜观察。Cd-6和Cd-8组血浆ET-1浓度均显著升高，且呈累积剂量依赖性。镉处理的大鼠主动脉内皮细胞中的韦伯-帕拉德（WP）体的数量增加，和脱粒和胞吐的WP机构专门发生在Cd-8组。ET-1的免疫反应优先定位于镉处理组和对照组的WP体，而镉处理组的粗面内质网仅。反应性也被发现在WP机构进行胞吐在Cd-8组。镉中毒诱导大鼠主动脉内皮细胞内ET-1-WP小体数量增加。通过胞吐作用增强其内容物的细胞外释放导致血浆ET-1浓度升高。
Male rats received daily intraperitoneal injections of cadmium sulphate (2.0 mg/kg) for 3 (Cd-3 group), 6 (Cd-6 group) and 8 days (Cd-8 group). The blood samples were prepared for endothelin (ET)-1 assay, and the thoracic aorta was investigated by both electron microscopy and immunoelectron microscopy using anti ET-1 sera. The plasma ET-1 concentrations of both Cd-6 and Cd-8 groups increased significantly in a cumulative dose-dependent manner. The cadmium-treated rat aorta showed an increase in the number of Weibel-Palade (WP) bodies in endothelial cells, and degranulation and exocytosis of WP bodies occurred exclusively in the Cd-8 group. Immunoreaction for ET-1 was localized preferentially in WP bodies of both cadmium-treated and control groups, and in the rough endoplasmic reticulum of the cadmium-treated groups only. Reactivity was also found on the WP bodies undergoing exocytosis in the Cd-8 group. Cadmium intoxication induces an increase in number of ET-1-storing WP bodies in the rat aorta endothelium. The enhancement of extracellular release of their contents by exocytosis results in elevation of the plasma ET-1 concentration.
通过放射免疫测定确定内毒素在体内和体外刺激内皮素释放。
DOI：
10.1016/0006-291x(89)91372-7
发表时间：
1989
影响因子：
3.1
作者：
Sugiura,M;Inagami,T;Kon,V
通讯作者：
Kon,V
影响因子：
15.9
作者：
MCEVER, RP;BECKSTEAD, JH;BAINTON, DF
通讯作者：
BAINTON, DF
影响因子：
19.6
作者：
KON, V;SUGIURA, M;HOOVER, RL
通讯作者：
HOOVER, RL