Differential gene expression is not required for facultative sex allocation: a transcriptome analysis of brain tissue in the parasitoid wasp Nasonia vitripennis.
Differential gene expression is not required for facultative sex allocation: a transcriptome analysis of brain tissue in the parasitoid wasp Nasonia vitripennis.
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DOI：
10.1098/rsos.171718
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发表时间：
2018-03
影响因子：
3.5
通讯作者：
Shuker DM
中科院分区：
文献类型：
--
作者：
Cook N;Boulton RA;Green J;Trivedi U;Tauber E;Pannebakker BA;Ritchie MG;Shuker DM
全转录组技术已被广泛用于行为遗传学，以识别与行为表现相关的基因，并提供其机制基础的线索。在这里，我们认为性分配行为的遗传基础的寄生蜂Nasonia vitripennis。雌性纳苏丽鱼后代的性别比例变化符合汉密尔顿的局部配偶竞争理论（LMC）。一只雌性或“创始人”在一块土地上产卵，将产生足够的儿子来使她的女儿受精。随着在一个补丁上产卵的“创始人”数量的增加（LMC下降），女性产生越来越多的男性偏见的性别比例。表型研究揭示了女性用来估计其儿子将经历的LMC水平的线索，但我们对性别分配背后的遗传学的理解是有限的。在这里，我们将雌性暴露于三种基础数量条件，即三种LMC条件，并允许它们产卵。只从这些雌性的头部提取mRNA以靶向脑组织。随后的RNA测序实验证实，差异基因表达与对性别分配线索的反应无关，我们必须转向基础神经科学来揭示这种令人印象深刻的行为可塑性的基础。
Whole-transcriptome technologies have been widely used in behavioural genetics to identify genes associated with the performance of a behaviour and provide clues to its mechanistic basis. Here, we consider the genetic basis of sex allocation behaviour in the parasitoid wasp Nasonia vitripennis. Female Nasonia facultatively vary their offspring sex ratio in line with Hamilton's theory of local mate competition (LMC). A single female or ‘foundress’ laying eggs on a patch will lay just enough sons to fertilize her daughters. As the number of ‘foundresses’ laying eggs on a patch increases (and LMC declines), females produce increasingly male-biased sex ratios. Phenotypic studies have revealed the cues females use to estimate the level of LMC their sons will experience, but our understanding of the genetics underlying sex allocation is limited. Here, we exposed females to three foundress number conditions, i.e. three LMC conditions, and allowed them to oviposit. mRNA was extracted from only the heads of these females to target the brain tissue. The subsequent RNA-seq experiment confirmed that differential gene expression is not associated with the response to sex allocation cues and that we must instead turn to the underlying neuroscience to reveal the underpinnings of this impressive behavioural plasticity.
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影响因子：
2.6
作者：
Immonen, Elina;Snook, Rhonda R.;Ritchie, Michael G.
通讯作者：
Ritchie, Michael G.
影响因子：
56.9
作者：
Jinek, Martin;Chylinski, Krzysztof;Charpentier, Emmanuelle
通讯作者：
Charpentier, Emmanuelle
影响因子：
3.3
作者：
Pannebakker, Bart A.;Halligan, Daniel L.;West, Stuart A.
通讯作者：
West, Stuart A.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
4.4
作者：
Manfredini F;Brown MJ;Vergoz V;Oldroyd BP
通讯作者：
Oldroyd BP