Synergistic effect of bolus exposure to zinc oxide nanoparticles on bleomycin-induced secretion of pro-fibrotic cytokines without lasting fibrotic changes in murine lungs.
Synergistic effect of bolus exposure to zinc oxide nanoparticles on bleomycin-induced secretion of pro-fibrotic cytokines without lasting fibrotic changes in murine lungs.
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DOI：
10.3390/ijms16010660
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发表时间：
2014-12-30
影响因子：
5.6
通讯作者：
Ichihara S
中科院分区：
文献类型：
--
作者：
Wu W;Ichihara G;Hashimoto N;Hasegawa Y;Hayashi Y;Tada-Oikawa S;Suzuki Y;Chang J;Kato M;D'Alessandro-Gabazza CN;Gabazza EC;Ichihara S
氧化锌（ZnO）纳米颗粒广泛应用于各种产品中，对这种制造材料的安全性评价非常重要。本研究探讨了肺暴露于ZnO纳米颗粒在肺纤维化小鼠模型的炎症和纤维化的影响。博莱霉素（BLM）皮下持续输注诱导肺纤维化。将雌性C57 BL/6 Jcl小鼠分为BLM处理组和未处理组。在每个治疗组中，通过咽部抽吸将0、10、20或30 μg ZnO纳米颗粒递送到肺中。在给药后第10天或第14天采集支气管肺泡灌洗液（BALF）和肺样本。肺暴露于单团氧化锌纳米粒子导致严重的，但短暂的炎症浸润和增厚的肺泡隔在肺中，沿着的总细胞计数和分类细胞计数在BLAF。BALF中白细胞介素（IL）-1β和转化生长因子（TGF）-β水平分别在第10天和第14天升高。在第10天，检测BLM和ZnO暴露对BALF中IL-1β和单核细胞趋化蛋白（MCP）-1的协同作用。本研究证明了肺暴露于ZnO纳米颗粒和皮下输注BLM对肺中促纤维化细胞因子分泌的协同作用。
Zinc oxide (ZnO) nanoparticles are widely used in various products, and the safety evaluation of this manufactured material is important. The present study investigated the inflammatory and fibrotic effects of pulmonary exposure to ZnO nanoparticles in a mouse model of pulmonary fibrosis. Pulmonary fibrosis was induced by constant subcutaneous infusion of bleomycin (BLM). Female C57BL/6Jcl mice were divided into BLM-treated and non-treated groups. In each treatment group, 0, 10, 20 or 30 µg of ZnO nanoparticles were delivered into the lungs through pharyngeal aspiration. Bronchoalveolar lavage fluid (BALF) and the lungs were sampled at Day 10 or 14 after administration. Pulmonary exposure by a single bolus of ZnO nanoparticles resulted in severe, but transient inflammatory infiltration and thickening of the alveolar septa in the lungs, along with the increase of total and differential cell counts in BLAF. The BALF level of interleukin (IL)-1β and transforming growth factor (TGF)-β was increased at Day 10 and 14, respectively. At Day 10, the synergistic effect of BLM and ZnO exposure was detected on IL-1β and monocyte chemotactic protein (MCP)-1 in BALF. The present study demonstrated the synergistic effect of pulmonary exposure to ZnO nanoparticles and subcutaneous infusion of BLM on the secretion of pro-fibrotic cytokines in the lungs.
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影响因子：
3.1
作者：
Manali ED;Moschos C;Triantafillidou C;Kotanidou A;Psallidas I;Karabela SP;Roussos C;Papiris S;Armaganidis A;Stathopoulos GT;Maniatis NA
通讯作者：
Maniatis NA
影响因子：
3.8
作者：
Bermudez, E;Mangum, JB;Everitt, JI
通讯作者：
Everitt, JI
DOI：
10.1164/rccm.201002-0185oc
发表时间：
2010-12-01
影响因子：
24.7
作者：
Kim, Yong Ho;Fazlollahi, Farnoosh;Crandall, Edward D.
通讯作者：
Crandall, Edward D.
影响因子：
2.5
作者：
Becheri, Alessio;Durr, Maximilian;Baglioni, Piero
通讯作者：
Baglioni, Piero
影响因子：
5.8
作者：
Braber S;Henricks PA;Nijkamp FP;Kraneveld AD;Folkerts G
通讯作者：
Folkerts G