Static and dynamic mechanics of the murine lung after intratracheal bleomycin.
Static and dynamic mechanics of the murine lung after intratracheal bleomycin.
复制标题
DOI：
10.1186/1471-2466-11-33
复制
发表时间：
2011-05-31
影响因子：
3.1
通讯作者：
Maniatis NA
中科院分区：
文献类型：
--
作者：
Manali ED;Moschos C;Triantafillidou C;Kotanidou A;Psallidas I;Karabela SP;Roussos C;Papiris S;Armaganidis A;Stathopoulos GT;Maniatis NA
尽管博来霉素小鼠模型广泛用于肺纤维化研究，但尚未使用新技术从肺功能的角度进行彻底验证。本研究的目的是系统地评估肺纤维化剂博莱霉素诱导小鼠肺功能的变化，并比较强迫振荡技术与准静态压力-容积曲线在博莱霉素暴露后的小鼠。向C57 BL/6（n = 40）和Balb/c（n = 32）小鼠单次腹膜内注射生理盐水（50 μL）或博来霉素（2 mg/Kg，溶于50 μL生理盐水）。分别在7天和21天（炎症和纤维化阶段）后测定损伤/纤维化评分、组织体积密度（TVD）、胶原含量、气道阻力（RN）、组织阻尼（G）和弹性系数（H）、气道阻力（η）和压力-容积曲线面积（PV-A）。使用单向方差分析和LSD事后检验进行统计假设检验。C57 BL/6和Balb/c小鼠在博来霉素后均出现体重减轻和肺部炎症。然而，只有C57 BL/6小鼠表现出恶病质和纤维化，表现为纤维化评分、TVD和胶原蛋白增加。在第7天，与盐水对照相比，在博来霉素暴露的C57 BL/6小鼠中PV-A显著增加，G和H非显著增加，并且在第21天记录到所有参数的进一步增加。根据组织学、TVD和胶原蛋白，G和H（而非PV-A）与纤维化的存在相关性良好。在Balb/c小鼠中，博莱霉素暴露后，胶原蛋白含量、组织学评分、TVD、H和G没有变化，但PV-A与生理盐水对照组相比显著增加。博莱霉素模型中的肺功能障碍在纤维化阶段比炎症阶段更明显。强迫振荡力学是实验性博来霉素诱导的肺纤维化的准确指标。在检测炎症和纤维化时，准静态PV曲线可能比强制振荡更敏感。
Despite its widespread use in pulmonary fibrosis research, the bleomycin mouse model has not been thoroughly validated from a pulmonary functional standpoint using new technologies. Purpose of this study was to systematically assess the functional alterations induced in murine lungs by fibrogenic agent bleomycin and to compare the forced oscillation technique with quasi-static pressure-volume curves in mice following bleomycin exposure. Single intratracheal injections of saline (50 μL) or bleomycin (2 mg/Kg in 50 μL saline) were administered to C57BL/6 (n = 40) and Balb/c (n = 32) mice. Injury/fibrosis score, tissue volume density (TVD), collagen content, airway resistance (RN), tissue damping (G) and elastance coefficient (H), hysteresivity (η), and area of pressure-volume curve (PV-A) were determined after 7 and 21 days (inflammation and fibrosis stage, respectively). Statistical hypothesis testing was performed using one-way ANOVA with LSD post hoc tests. Both C57BL/6 and Balb/c mice developed weight loss and lung inflammation after bleomycin. However, only C57BL/6 mice displayed cachexia and fibrosis, evidenced by increased fibrosis score, TVD, and collagen. At day 7, PV-A increased significantly and G and H non-significantly in bleomycin-exposed C57BL/6 mice compared to saline controls and further increase in all parameters was documented at day 21. G and H, but not PV-A, correlated well with the presence of fibrosis based on histology, TVD and collagen. In Balb/c mice, no change in collagen content, histology score, TVD, H and G was noted following bleomycin exposure, yet PV-A increased significantly compared to saline controls. Lung dysfunction in the bleomycin model is more pronounced during the fibrosis stage rather than the inflammation stage. Forced oscillation mechanics are accurate indicators of experimental bleomycin-induced lung fibrosis. Quasi-static PV-curves may be more sensitive than forced oscillations at detecting inflammation and fibrosis.
登录
查看更多内容
DOI：
10.1152/ajplung.00170.2004
发表时间：
2004-12-01
影响因子：
4.9
作者：
Cavarra, E;Carraro, F;Lungarella, G
通讯作者：
Lungarella, G
影响因子：
4.6
作者：
Lemay, AM;Haston, CK
通讯作者：
Haston, CK
DOI：
10.1164/ajrccm.162.4.9912011
发表时间：
2000-10-01
影响因子：
24.7
作者：
Ebihara, T;Venkatesan, N;Ludwig, MS
通讯作者：
Ludwig, MS
DOI：
10.1152/ajplung.00492.2005
发表时间：
2006-08-01
影响因子：
4.9
作者：
Lovgren, Alysia Kern;Jania, Leigh A.;Koller, Beverly H.
通讯作者：
Koller, Beverly H.
影响因子：
3.3
作者：
HANTOS, Z;DAROCZY, B;NAGY, S
通讯作者：
NAGY, S