Characterization of the Antioxidant Effects of γ-Oryzanol: Involvement of the Nrf2 Pathway.
Characterization of the Antioxidant Effects of γ-Oryzanol: Involvement of the Nrf2 Pathway.
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DOI：
10.1155/2018/2987249
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发表时间：
2018
影响因子：
--
通讯作者：
Uberti D
中科院分区：
文献类型：
--
作者：
Rungratanawanich W;Abate G;Serafini MM;Guarienti M;Catanzaro M;Marziano M;Memo M;Lanni C;Uberti D
γ-谷维素（ORY）因其抗氧化潜力而闻名。然而，ORY发挥其抗氧化作用的机制仍不清楚。在本文中，ORY的抗氧化特性进行了研究，其潜在的影响，作为一个活性氧和氮（ROS/RNS）清除剂，并在激活抗氧化促进细胞内途径，利用人胚肾细胞（HEK-293）。24 h ORY暴露显著抑制H2 O2-诱导的ROS/RNS产生，这种作用在3 h后可持续至少24 h。ORY预处理还增强了抗氧化酶：超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPX）的活性。有趣的是，ORY诱导核因子（红细胞衍生2）样2（Nrf 2）核转位和上调Nrf 2依赖性防御基因，如NAD（P）H醌还原酶（NQO 1），血红素加氧酶-1（HO-1），谷胱甘肽合成酶（GSS）在mRNA和蛋白水平在基础条件下和H2 O2损伤后。因此，这项研究表明ORY在调节Nrf 2通路中具有有趣的作用，该通路也参与调节寿命以及年龄相关疾病。
γ-Oryzanol (ORY) is well known for its antioxidant potential. However, the mechanism by which ORY exerts its antioxidant effect is still unclear. In this paper, the antioxidant properties of ORY were investigated for its potential effects as a reactive oxygen and nitrogen species (ROS/RNS) scavenger and in activating antioxidant-promoting intracellular pathways utilizing the human embryonic kidney cells (HEK-293). The 24 h ORY exposure significantly prevented hydrogen peroxide- (H2O2-) induced ROS/RNS production at 3 h, and this effect was sustained for at least 24 h. ORY pretreatment also enhanced the activity of antioxidant enzymes: superoxide dismutase (SOD) and glutathione peroxidase (GPX). Interestingly, ORY induced the nuclear factor (erythroid-derived 2)-like 2 (Nrf2) nuclear translocation and upregulation of Nrf2-dependent defensive genes such as NAD(P)H quinone reductase (NQO1), heme oxygenase-1 (HO-1), and glutathione synthetase (GSS) at mRNA and protein levels in both basal condition and after H2O2 insult. Thus, this study suggested an intriguing effect of ORY in modulating the Nrf2 pathway, which is also involved in regulating longevity as well as age-related diseases.
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影响因子：
5.6
作者：
Ismail N;Ismail M;Farhana Fathy S;Asma Musa SN;Umar Imam M;Foo JB;Iqbal S
通讯作者：
Iqbal S
影响因子：
2.8
作者：
Dinu, Diana;Bodea, Gabriela Oana;Dinischiotu, Anca
通讯作者：
Dinischiotu, Anca
影响因子：
8.8
作者：
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通讯作者：
Siriamornpun, Sirithon
DOI：
10.1006/bbrc.1997.6943
发表时间：
1997-07-18
影响因子：
3.1
作者：
Itoh, K;Chiba, T;Nabeshima, Y
通讯作者：
Nabeshima, Y
影响因子：
4.1
作者：
AWASTHI, YC;DAO, DD;SRIVASTAVA, SK
通讯作者：
SRIVASTAVA, SK