Neuroprotective effects of germinated brown rice against hydrogen peroxide induced cell death in human SH-SY5Y cells.
Neuroprotective effects of germinated brown rice against hydrogen peroxide induced cell death in human SH-SY5Y cells.
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DOI：
10.3390/ijms13089692
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发表时间：
2012
影响因子：
5.6
通讯作者：
Iqbal S
中科院分区：
文献类型：
--
作者：
Ismail N;Ismail M;Farhana Fathy S;Asma Musa SN;Umar Imam M;Foo JB;Iqbal S
本文研究了发芽糙米（GBR）、糙米（BR）和γ-氨基丁酸（GABA）对过氧化氢（H_2O_2）诱导的人神经母细胞瘤SH-SY 5 Y细胞凋亡的神经保护和抗氧化作用。结果表明，GBR能抑制H2 O2对SH-SY 5 Y细胞的毒性作用，并使细胞周期阻滞于G 0/G1期。此外，GBR降低了SH-SY 5 Y细胞的线粒体膜电位（MMP），并阻止了磷脂酰丝氨酸（PS）易位，这是细胞凋亡和随后细胞死亡的关键特征。GBR表现出更好的神经保护和抗氧化活性相比，BR和GABA。这些结果表明，GBR具有高的抗氧化活性，并通过阻断细胞周期的重新进入和凋亡机制抑制SH-SY 5 Y细胞的细胞死亡。因此，GBR可开发为预防氧化应激和细胞凋亡引起的神经退行性疾病的增值功能性食品。
The neuroprotective and antioxidative effects of germinated brown rice (GBR), brown rice (BR) and commercially available γ-aminobutyric acid (GABA) against cell death induced by hydrogen peroxide (H2O2) in human neuroblastoma SH-SY5Y cells have been investigated. Results show that GBR suppressed H2O2-mediated cytotoxicity and induced G0/G1 phase cell cycle arrest in SH-SY5Y cells. Moreover, GBR reduced mitochondrial membrane potential (MMP) and prevented phosphatidylserine (PS) translocation in SH-SY5Y cells, key features of apoptosis, and subsequent cell death. GBR exhibited better neuroprotective and antioxidative activities as compared to BR and GABA. These results indicate that GBR possesses high antioxidative activities and suppressed cell death in SH-SY5Y cells by blocking the cell cycle re-entry and apoptotic mechanisms. Therefore, GBR could be developed as a value added functional food to prevent neurodegenerative diseases caused by oxidative stress and apoptosis.
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影响因子：
5
作者：
Ruffels, J;Griffin, M;Dickenson, JM
通讯作者：
Dickenson, JM
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