Localization and targeting of voltage-dependent ion channels in mammalian central neurons.
Localization and targeting of voltage-dependent ion channels in mammalian central neurons.
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DOI：
10.1152/physrev.00002.2008
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发表时间：
2008-10
影响因子：
33.6
通讯作者：
Trimmer JS
中科院分区：
文献类型：
--
作者：
Vacher H;Mohapatra DP;Trimmer JS
神经元的固有电学性质和突触输入输出关系受电压依赖性离子通道的作用支配。具有不同功能特性的离子通道在神经元不连续部位的特定群体的定位显著影响兴奋性和突触传递。分子克隆研究揭示了编码电压依赖性离子通道主亚基和辅助亚单位的基因大家族，其中大部分在哺乳动物中枢神经元中表达。最近的工作集中在确定这些亚基中的哪些共同组装成天然的神经元通道复合体，以及这些复合体的细胞和亚细胞分布，作为理解这些通道对神经元功能的特定方面的贡献的关键一步。在这里，我们回顾了最近的研究进展，旨在用原位杂交和免疫组织化学方法确定哺乳动物脑神经元中特定离子通道亚单位的细胞和亚细胞分布。我们还讨论了离子通道亚基在特定神经元隔间的谱系以及对神经元生理学的影响。最后，我们讨论了确定许多神经元离子通道观察到的离散亚细胞分布的新机制。
The intrinsic electrical properties and the synaptic input-output relationships of neurons are governed by the action of voltage-dependent ion channels. The localization of specific population of ion channels with distinct functional properties at discrete sites in neurons dramatically impacts excitability and synaptic transmission. Molecular cloning studies have revealed a large family of genes encoding voltage-dependent ion channel principal and auxiliary subunits, most of which are expressed in mammalian central neurons. Much recent effort has focused on determining which of these subunits co-assemble into native neuronal channel complexes, and the cellular and subcellular distributions of these complexes, as a crucial step in understanding the contribution of these channels to specific aspects of neuronal function. Here we review progress made on recent studies aimed at determining the cellular and subcellular distribution of specific ion channel subunits in mammalian brain neurons using in situ hybridization, and immunohistochemistry. We also discuss the repertoire of ion channel subunits in specific neuronal compartments and implications for neuronal physiology. Finally, we discuss the emerging mechanisms for determining the discrete subcellular distributions observed for many neuronal ion channels.
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影响因子：
14.9
作者：
Bruford, Elspeth A.;Lush, Michael J.;Wright, Mathew W.;Sneddon, Tam P.;Povey, Sue;Birney, Ewan
通讯作者：
Birney, Ewan
影响因子：
16.2
作者：
BALDWIN, TJ;TSAUR, ML;JAN, LY
通讯作者：
JAN, LY
影响因子：
25
作者：
Baranauskas, G;Tkatch, T;Surmeier, DJ
通讯作者：
Surmeier, DJ
影响因子：
1.7
作者：
ALBENSI, BC;RYUJIN, KT;FILLOUS, F
通讯作者：
FILLOUS, F
影响因子：
3.3
作者：
Antonucci, DE;Lim, ST;Trimmer, JS
通讯作者：
Trimmer, JS