Viscoelastic and equilibrium shear properties of human meniscus: Relationships with tissue structure and composition.
Viscoelastic and equilibrium shear properties of human meniscus: Relationships with tissue structure and composition.
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DOI：
10.1016/j.jbiomech.2021.110343
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发表时间：
2021-05-07
影响因子：
2.4
通讯作者：
Travascio F
中科院分区：
文献类型：
--
作者：
Norberg C;Filippone G;Andreopoulos F;Best TM;Baraga M;Jackson AR;Travascio F
半月板在维持膝关节功能和保护关节免受继发性病变（包括骨关节炎）方面至关重要。虽然人体骨的大部分力学性质已被表征，但据我们所知，其动态剪切性质从未有过报道。此外，关于组织结构和组成的剪切特性知之甚少。这对于理解骨骼损伤的机制以及在再生医学中设计和开发模仿天然组织的组织工程支架至关重要。因此，本研究的目的是表征人类半月板的动态和平衡剪切特性与其各向异性和组成。从内侧和外侧椎间盘的轴向和周向解剖平面制备标本。频率扫描和应力松弛试验得出储能模量（G '）和损耗模量（G“）以及平衡剪切模量（G”）。模量与水，糖胺聚糖（GAGs），胶原蛋白含量的相关性进行了研究。半月板表现出粘弹性行为。动态剪切性能与组织成分有关：G '、G“和G与组织含水量呈负相关; G'和G”与GAG和胶原蛋白呈正相关（仅在环周样本中）。胶原纤维垂直于剪切面的环向试样表现出较好的上级动态力学性能，其G' ~70 kPa和G“~ 10 kPa分别比轴向试样的G'~15 kPa和G”~ 1 kPa高。纤维取向不影响G值，其范围为~50 ~~ 100 kPa。
The meniscus is crucial in maintaining the knee function and protecting the joint from secondary pathologies, including osteoarthritis. Although most of the mechanical properties of human menisci have been characterized, to our knowledge, its dynamic shear properties have never been reported. Moreover, little is known about meniscal shear properties in relation to tissue structure and composition. This is crucial to understand mechanisms of meniscal injury, as well as, in regenerative medicine, for the design and development of tissue engineered scaffolds mimicking the native tissue. Hence, the objective of this study was to characterize the dynamic and equilibrium shear properties of human meniscus in relation to its anisotropy and composition. Specimens were prepared from the axial and the circumferential anatomical planes of medial and lateral menisci. Frequency sweeps and stress relaxation tests yielded storage (G’) and loss moduli (G’’), and equilibrium shear modulus (G). Correlations of moduli with water, glycosaminoglycans (GAGs), and collagen content were investigated. The meniscus exhibited viscoelastic behavior. Dynamic shear properties were related to tissue composition: negative correlations were found between G’, G’’ and G, and meniscal water content; positive correlations were found for G’ and G’’ with GAG and collagen (only in circumferential samples). Circumferential samples, with collagen fibers orthogonal to the shear plane, exhibited superior dynamic mechanical properties, with G’ ~70 kPa and G’’ ~10kPa, compared to those of the axial plane ~15 kPa and ~1kPa, respectively. Fiber orientation did not affect the values of G, which ranged from ~50 to ~100kPa.
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影响因子：
7
作者：
Burton-Wurster, N;Liu, W;Roughley, PJ
通讯作者：
Roughley, PJ
影响因子：
2.8
作者：
ANDERSON, DR;WOO, SLY;GERSHUNI, DH
通讯作者：
GERSHUNI, DH
DOI：
10.1243/0954411001535651
发表时间：
2000-01-01
影响因子：
1.8
作者：
Leslie, BW;Gardner, DL;Moran, RS
通讯作者：
Moran, RS
影响因子：
2.8
作者：
PROCTOR, CS;SCHMIDT, MB;MOW, VC
通讯作者：
MOW, VC
DOI：
10.1089/ten.teb.2008.0189
发表时间：
2009-03
期刊：
Tissue engineering. Part B, Reviews
影响因子：
--
作者：
Revell CM;Athanasiou KA
通讯作者：
Athanasiou KA