High levels of dietary stearate promote adiposity and deteriorate hepatic insulin sensitivity.
High levels of dietary stearate promote adiposity and deteriorate hepatic insulin sensitivity.
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DOI：
10.1186/1743-7075-7-24
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发表时间：
2010-03-27
影响因子：
4.5
通讯作者：
van Dijk KW
中科院分区：
文献类型：
--
作者：
van den Berg SA;Guigas B;Bijland S;Ouwens M;Voshol PJ;Frants RR;Havekes LM;Romijn JA;van Dijk KW
对于特定饱和脂肪酸在高脂肪饮食引起的肥胖和胰岛素抵抗中的作用，我们所知相对较少。在这里，我们研究了硬脂酸盐在高脂肪饮食（45%的能量为脂肪）中对全身能量代谢和组织特异性胰岛素敏感性的影响。C57Bl/6小鼠饲喂以棕榈油为基础的低硬脂酸饲粮或两种富含硬脂酸的饲粮中的一种，一种以猪油为基础的饲粮，一种以棕榈油为基础的饲粮中添加三硬脂酸（达到猪油基础饲粮的硬脂酸水平），为期5周。第5周采用间接量热法测定随意喂养大鼠的氧化代谢。通过DEXA扫描分析评估体重和成分的变化。在第5周结束时，采用高胰岛素-正糖钳形分析和Western blot方法评估组织特异性胰岛素敏感性。间接量热分析显示，高水平的膳食硬脂酸导致较低的热量能量消耗，其特征是脂肪酸的氧化较低。与这种代谢表型一致，富含硬脂酸盐饮食的小鼠获得了更多的脂肪组织质量。通过高胰岛素-正糖钳夹和胰岛素诱导的PKBser473磷酸化分析来评估全身和组织特异性胰岛素敏感性。所有高脂肪饮食都降低了全身胰岛素敏感性。然而，尽管所有高脂饮食都损害了胰岛素刺激的外周组织葡萄糖摄取，但肝脏胰岛素敏感性仅受到富含硬脂酸盐饮食的影响。肝脏和骨骼肌中胰岛素诱导的PKBser473磷酸化的类似损伤证实了这种胰岛素敏感性降低的组织特异性模式。在C57Bl/6小鼠中，5周富含硬脂酸盐的高脂肪饮食诱导代谢状态倾向于低氧化代谢，脂肪增加和以严重肝脏胰岛素抵抗为特征的全身胰岛素抵抗。上述结果表明，决定高脂喂养C57Bl/6小鼠肝脏胰岛素敏感性的不是饲料脂肪含量，而是饲料脂肪酸组成。
Relatively little is known about the role of specific saturated fatty acids in the development of high fat diet induced obesity and insulin resistance. Here, we have studied the effect of stearate in high fat diets (45% energy as fat) on whole body energy metabolism and tissue specific insulin sensitivity. C57Bl/6 mice were fed a low stearate diet based on palm oil or one of two stearate rich diets, one diet based on lard and one diet based on palm oil supplemented with tristearin (to the stearate level of the lard based diet), for a period of 5 weeks. Ad libitum fed Oxidative metabolism was assessed by indirect calorimetry at week 5. Changes in body mass and composition was assessed by DEXA scan analysis. Tissue specific insulin sensitivity was assessed by hyperinsulinemic-euglycemic clamp analysis and Western blot at the end of week 5. Indirect calorimetry analysis revealed that high levels of dietary stearate resulted in lower caloric energy expenditure characterized by lower oxidation of fatty acids. In agreement with this metabolic phenotype, mice on the stearate rich diets gained more adipose tissue mass. Whole body and tissue specific insulin sensitivity was assessed by hyperinsulinemic-euglycemic clamp and analysis of insulin induced PKBser473 phosphorylation. Whole body insulin sensitivity was decreased by all high fat diets. However, while insulin-stimulated glucose uptake by peripheral tissues was impaired by all high fat diets, hepatic insulin sensitivity was affected only by the stearate rich diets. This tissue-specific pattern of reduced insulin sensitivity was confirmed by similar impairment in insulin-induced phosphorylation of PKBser473 in both liver and skeletal muscle. In C57Bl/6 mice, 5 weeks of a high fat diet rich in stearate induces a metabolic state favoring low oxidative metabolism, increased adiposity and whole body insulin resistance characterized by severe hepatic insulin resistance. These results indicate that dietary fatty acid composition per sé rather than dietary fat content determines insulin sensitivity in liver of high fat fed C57Bl/6 mice.
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影响因子：
3.2
作者：
van den Hoek, A. M.;Teusink, B.;Pijl, H.
通讯作者：
Pijl, H.
影响因子：
3.6
作者：
LEYTON, J;DRURY, PJ;CRAWFORD, MA
通讯作者：
CRAWFORD, MA
影响因子：
6.5
作者：
Goudriaan, JR;Dahlmans, VEH;Voshol, PJ
通讯作者：
Voshol, PJ
影响因子：
8.8
作者：
Nkondjock, A;Krewski, D;Ghadirian, P
通讯作者：
Ghadirian, P
影响因子：
1.9
作者：
Pai, T;Yeh, YY
通讯作者：
Yeh, YY