Using lipidomics to reveal details of lipid accumulation in developing seeds from oilseed rape (Brassica napus L.).
Using lipidomics to reveal details of lipid accumulation in developing seeds from oilseed rape (Brassica napus L.).
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DOI：
10.1016/j.bbalip.2017.12.010
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发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
Harwood JL
中科院分区：
文献类型：
--
作者：
Woodfield HK;Cazenave-Gassiot A;Haslam RP;Guschina IA;Wenk MR;Harwood JL
随着可用农业用地的减少，同时对石油的需求增加，目前人们对提高油料作物的生产力很感兴趣。我们一直致力于解决这个问题，通过研究油菜（Brassica napus L）油积累的调控。作为这项研究的一部分，我们对发育中的种子进行了详细的脂质组学分析。在个别脂质类的分子物种分布揭示了相当不同的模式，并显示代谢连接是重要的。随着种子的发育，分子种类的分布发生了变化，尤其是在油分积累的早期（花后20天，27天）。利用二酰基甘油、磷脂酰胆碱和酰基辅酶A分子种类的模式预测了二酰基甘油酰基转移酶（DGAT）和磷脂：二酰基甘油酰基转移酶对三酰基甘油产生的可能相对贡献。我们的计算表明，DGAT可能持有更重要的作用，在影响TAG的分子组成。酶的选择性对最终的分子物种模式有重要影响。我们的数据大大有助于我们了解世界第三大油料作物中的脂质积累。本文报道了油菜种子发育过程中的脂类组学分析，结果表明，不同种类的脂类在发育过程中存在着明显的差异。
With dwindling available agricultural land, concurrent with increased demand for oil, there is much current interest in raising oil crop productivity. We have been addressing this issue by studying the regulation of oil accumulation in oilseed rape (Brassica napus L). As part of this research we have carried out a detailed lipidomic analysis of developing seeds. The molecular species distribution in individual lipid classes revealed quite distinct patterns and showed where metabolic connections were important. As the seeds developed, the molecular species distributions changed, especially in the period of early (20 days after flowering, DAF) to mid phase (27DAF) of oil accumulation. The patterns of molecular species of diacylglycerol, phosphatidylcholine and acyl-CoAs were used to predict the possible relative contributions of diacylglycerol acyltransferase (DGAT) and phospholipid:diacylglycerol acyltransferase to triacylglycerol production. Our calculations suggest that DGAT may hold a more important role in influencing the molecular composition of TAG. Enzyme selectivity had an important influence on the final molecular species patterns. Our data contribute significantly to our understanding of lipid accumulation in the world's third most important oil crop. Lipidomic analysis of developing rapeseed seeds is reported Results show distinct differences between lipid classes Changes in molecular species distributions were found during development The data were used to evaluate the contribution of different synthetic pathways
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影响因子：
4.3
作者：
Banaś W;Sanchez Garcia A;Banaś A;Stymne S
通讯作者：
Stymne S
DOI：
10.1073/pnas.1318511111
发表时间：
2014-01-21
影响因子：
11.1
作者：
Bates, Philip D.;Johnson, Sean R.;Browse, John
通讯作者：
Browse, John
DOI：
10.1016/j.bbalip.2016.03.021
发表时间：
2016-09-01
影响因子：
4.8
作者：
Bates, Philip D.
通讯作者：
Bates, Philip D.
影响因子：
11.6
作者：
DEWEY, RE;WILSON, RF;GOODE, JH
通讯作者：
GOODE, JH
DOI：
10.1073/pnas.120067297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Dahlqvist, A;St책hl, U;Stymne, H
通讯作者：
Stymne, H