Molecular basis for the heterogeneity of human tyrosinase.
Molecular basis for the heterogeneity of human tyrosinase.
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人酪氨酸酶异质性的分子基础。
DOI：
10.1620/tjem.156.403
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
T. Cohen
中科院分区：
文献类型：
--
作者：
S. Shibahara;Y. Tomita;H. Tagami;R. Müller;T. Cohen
代表人酪氨酸酶mRNA的cDNA克隆pHT γ 1是通过用与人酪氨酸酶cDNA片段Pmel 34互补的合成寡核苷酸筛选黑素瘤cDNA文库而分离的[Kwon等（1987）Proc.nat.Acad. Sci. USA 84，7473-7477]。然而，在两种cDNA pHT γ 1和Pmel 34之间的核苷酸序列中存在许多差异，特别是在编码酶羧基末端的区域（推定的外显子5）中。因此，我们克隆的基因组DNA片段携带的外显子5的人酪氨酸酶基因，通过筛选人胎盘基因组DNA文库与克隆的cDNA探针。测定了人酪氨酸酶cDNA及其部分基因的核苷酸序列。成熟的人酪氨酸酶由511个氨基酸组成，分子量为58，000。我们提供的证据表明，在人类色素性黑色素瘤细胞中存在至少两种人酪氨酸酶mRNA的选择性剪接产生的。
A cDNA clone, pHT gamma 1, representing human tyrosinase mRNA was isolated by screening a melanoma cDNA library with a synthetic oligonucleotide complementary to a segment of the human tyrosinase cDNA, Pmel 34 [Kwon et al. (1987) Proc. nat. Acad. Sci. USA 84, 7473-7477]. However, there are a number of differences in the nucleotide sequence between two cDNAs, pHT gamma 1 and Pmel 34, particularly in the region coding for the carboxyl terminus of the enzyme (putative exon 5). We therefore cloned the genomic DNA segment carrying the exon 5 of the human tyrosinase gene by screening a human placental genomic DNA library with a cloned cDNA probe. The nucleotide sequences of human tyrosinase cDNA as well as part of its gene were determined. Mature human tyrosinase is composed of 511 amino acids with a molecular weight of 58,000. We provide evidence for the presence of at least two species of human tyrosinase mRNA generated by alternative splicing in human pigmented melanoma cells.
DOI：
10.1016/s0006-291x(88)81370-6
发表时间：
1988-06-30
影响因子：
3.1
作者：
KWON, BS;WAKULCHIK, M;KESTLER, D
通讯作者：
KESTLER, D
DOI：
10.1073/pnas.84.21.7473
发表时间：
1987-11-01
影响因子：
11.1
作者：
KWON, BS;HAQ, AK;HALABAN, R
通讯作者：
HALABAN, R