Activity Maintenance Characteristics and Protease Adsorption on Langmuir Monolayer of Organo‐Modified Single‐Walled Carbon Nanotubes

Activity Maintenance Characteristics and Protease Adsorption on Langmuir Monolayer of Organo‐Modified Single‐Walled Carbon Nanotubes
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有机改性单壁碳纳米管Langmuir单层的活性维持特性及蛋白酶吸附

DOI:
10.1002/slct.202100862
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发表时间:
2021
期刊:
影响因子:
2.1
通讯作者:
Fujimori Atsuhio
Fujimori Atsuhio
中科院分区:
化学4区
文献类型:
--
作者:
Almarasy Ahmed A.;Yamada Yuna;Mashiyama Yuki;Maruyama Haruka;Kimura Yusuke;Fujimori Atsuhio

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The activity maintenance characteristics of proteases adsorbed and immobilized on a monolayer of organo‐modified single‐walled carbon nanotubes (SWCNTs) were evaluated in this study. After forming a monolayer on the water surface of the organo‐modified SWCNTs, protease was introduced into the subphase, and monolayer immobilization was attained by post‐adsorption. The protease‐adsorbed organo‐SWCNT multilayers were immersed in an aqueous solution of luminescent casein, and fluorescence emission was observed. Proteases had cleaved the molecular chain of luminescent casein, which causes luminescence. Although the protease in the aqueous solution could cleave the molecular chain only up to approximately room temperature, the protease immobilized on the solid substrate could cleave the chain up to 160 °C, indicating sharp emission spectra.
通过扫描隧道显微镜观察到石墨上有序的蛋白质阵列
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