Imaging of Her2-targeted magnetic nanoparticles for breast cancer detection: comparison of SQUID-detected magnetic relaxometry and MRI.
Imaging of Her2-targeted magnetic nanoparticles for breast cancer detection: comparison of SQUID-detected magnetic relaxometry and MRI.
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DOI：
10.1002/cmmi.499
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发表时间：
2012-05
影响因子：
--
通讯作者：
Flynn, Edward R.
中科院分区：
文献类型：
--
作者：
Adolphi, Natalie L.;Butler, Kimberly S.;Lovato, Debbie M.;Tessier, T. E.;Trujillo, Jason E.;Hathaway, Helen J.;Fegan, Danielle L.;Monson, Todd C.;Stevens, Tyler E.;Huber, Dale L.;Ramu, Jaivijay;Milne, Michelle L.;Altobelli, Stephen A.;Bryant, Howard C.;Larson, Richard S.;Flynn, Edward R.
关键词：
磁弛豫法和磁共振成像(MRI)都可以用来检测和定位目标磁性纳米颗粒，非侵入性和无电离辐射。磁弛豫法在其特异性(只检测到纳米颗粒)和弛豫信号与纳米颗粒数量的线性相关性方面具有优势。在这项研究中，比较了超导量子干涉装置(SQUID)检测的磁弛豫法和标准的4.7T磁共振成像对单核氧化铁纳米颗粒的检测。将纳米颗粒与Her2单抗偶联，并靶向于表达Her2的MCF7/Her2-18乳腺癌细胞)；通过磁弛豫法和铁试剂法评估纳米颗粒与细胞的结合。同样的纳米颗粒标记细胞，经过连续稀释，被用来评估检测下限和磁共振弛豫度。磁弛豫法的检测下限为125,000个纳米颗粒标记细胞，位于距鱿鱼传感器3厘米处。T2加权磁共振成像在150μ的L体积中产生15,600个细胞，r1=1.1 mM−1s−1，r2=166 mM−1s−1。将HER2靶向纳米颗粒直接注射到裸鼠MCF7/HER2-18移植瘤中，进行磁弛豫成像和4.7T磁共振成像，使这两种技术能够直接进行比较。松弛测量图像和小鼠核磁共振图像的联合配准结果很好地吻合。本文还介绍了一种用弛豫计量法准确测定肿瘤和肝脏中铁含量的方法。这些结果证明了SQUID检测的磁弛豫成像在乳腺癌的特异性检测和基于磁性纳米颗粒的治疗监测方面的潜力。
Both magnetic relaxometry and magnetic resonance imaging (MRI) can be used to detect and locate targeted magnetic nanoparticles, non-invasively and without ionizing radiation. Magnetic relaxometry offers advantages in terms of its specificity (only nanoparticles are detected) and the linear dependence of the relaxometry signal on the number of nanoparticles present. In this study, detection of single-core iron oxide nanoparticles by Superconducting Quantum Interference Device (SQUID)-detected magnetic relaxometry and standard 4.7 T MRI are compared. The nanoparticles were conjugated to a Her2 monoclonal antibody and targeted to Her2-expressing MCF7/Her2-18 breast cancer cells); binding of the nanoparticles to the cells was assessed by magnetic relaxometry and iron assay. The same nanoparticle-labeled cells, serially diluted, were used to assess the detection limits and MR relaxivities. The detection limit of magnetic relaxometry was 125,000 nanoparticle-labeled cells at 3 cm from the SQUID sensors. T2-weighted MRI yielded a detection limit of 15,600 cells in a 150 μl volume, with r1 = 1.1 mM−1s−1 and r2 = 166 mM−1s−1. Her2-targeted nanoparticles were directly injected into xenograft MCF7/Her2-18 tumors in nude mice, and magnetic relaxometry imaging and 4.7 T MRI were performed, enabling direct comparison of the two techniques. Co-registration of relaxometry images and MRI of mice resulted in good agreement. A method for obtaining accurate quantification of microgram quantities of iron in the tumors and liver by relaxometry was also demonstrated. These results demonstrate the potential of SQUID-detected magnetic relaxometry imaging for the specific detection of breast cancer and the monitoring of magnetic nanoparticle-based therapies.
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