Identification of microspore-active promoters that allow targeted manipulation of gene expression at early stages of microgametogenesis in Arabidopsis.
Identification of microspore-active promoters that allow targeted manipulation of gene expression at early stages of microgametogenesis in Arabidopsis.
复制标题
鉴定拟南芥在微汞生成的早期阶段靶向操纵基因表达的小孢子活性启动子。
DOI：
10.1186/1471-2229-6-31
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发表时间：
2006-12-21
影响因子：
5.3
通讯作者：
Twell D
中科院分区：
文献类型：
--
作者：
Honys D;Oh SA;Renák D;Donders M;Solcová B;Johnson JA;Boudová R;Twell D
雄配子体发育的有效功能分析需要新的工具，使空间和时间控制的表达标记基因和修饰的基因的兴趣。特别地，需要在早期发育阶段（包括小孢子）驱动表达的启动子。转录组学数据集涵盖了拟南芥雄配子体发育的四个渐进阶段，用于选择显示雄配子体特异性的早期表达谱的候选基因。启动子-GUS报告基因分析的候选基因确定了三个启动子（MSP 1，MSP 2，和MSP 3），是活跃的小孢子，否则是特定的雄配子体和绒毡层。MSP 1和MSP 2启动子用于成功地补充和恢复配子体二合一（tio）突变体的雄性传递，该突变体在第一次小孢子分裂时是减数分裂缺陷的。我们证明了MSP启动子的有效应用，可以用来阐明配子体基因功能的小孢子在男性特定的方式作为工具。
The effective functional analysis of male gametophyte development requires new tools enabling the spatially and temporally controlled expression of both marker genes and modified genes of interest. In particular, promoters driving expression at earlier developmental stages including microspores are required. Transcriptomic datasets covering four progressive stages of male gametophyte development in Arabidopsis were used to select candidate genes showing early expression profiles that were male gametophyte-specific. Promoter-GUS reporter analysis of candidate genes identified three promoters (MSP1, MSP2, and MSP3) that are active in microspores and are otherwise specific to the male gametophyte and tapetum. The MSP1 and MSP2 promoters were used to successfully complement and restore the male transmission of the gametophytic two-in-one (tio) mutant that is cytokinesis-defective at first microspore division. We demonstrate the effective application of MSP promoters as tools that can be used to elucidate gametophytic gene functions in microspores in a male-specific manner.
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影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
7.2
作者：
Bate, N;Spurr, C;Twell, D
通讯作者：
Twell, D
影响因子：
11.6
作者：
Cheng, NH;Pittman, JK;Hirschi, KD
通讯作者：
Hirschi, KD
影响因子：
12.3
作者：
Honys D;Twell D
通讯作者：
Twell D
影响因子：
11.6
作者：
EADY, C;LINDSEY, K;TWELL, D
通讯作者：
TWELL, D