Transcriptome analysis of haploid male gametophyte development in Arabidopsis.
Transcriptome analysis of haploid male gametophyte development in Arabidopsis.
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DOI：
10.1186/gb-2004-5-11-r85
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发表时间：
2004
期刊：
影响因子：
12.3
通讯作者：
Twell D
中科院分区：
文献类型：
--
作者：
Honys D;Twell D
拟南芥雄配子体发育的转录组分析揭示了基因表达的不同时间类别，并为详细研究所涉及的调控途径打开了大门。开花植物的单倍体雄配子体世代由二细胞或三细胞花粉粒组成。这种功能特化被认为是显花植物进化成功的关键因素。此外，花粉个体发育也是一个有吸引力的模型，在其中剖析控制细胞生长，不对称细胞分裂和细胞分化的细胞网络。我们的目标是全面定义整个发育过程中的雄性单倍体转录组，这也是详细了解这些过程的重要一步。我们已经开发了拟南芥的分阶段孢子分离程序，并使用AffyesterATH1基因组阵列来识别总共13，977个雄性配子体表达的mRNA，其中9.7%是雄性配子体特异性的。从双细胞到三细胞花粉的过渡是伴随着不同的mRNA种类的数量下降和雄配子体特异性转录的比例增加。调控蛋白的表达谱和不同的共表达基因簇被确定为可能对应于配子体调控网络的组成部分。此外，转录组和实验数据的整合揭示了翻译因子的早期合成及其支持花粉管生长的需求。从增殖的小孢子到终末分化的花粉的进展的特征在于早期程序基因的大规模抑制和成熟花粉中独特的晚期基因表达程序的激活。这些数据提供了关于配子体转录的知识的量子增长，并为新的基因组主导的调控网络和细胞功能的研究奠定了基础，这些调控网络和细胞功能用于指定雄配子体发育。
A transcriptome analysis of male gametophyte development in Arabidopsis uncovers distinct temporal classes of gene expression and opens the door to detailed studies of the regulatory pathways involved. The haploid male gametophyte generation of flowering plants consists of two- or three-celled pollen grains. This functional specialization is thought to be a key factor in the evolutionary success of flowering plants. Moreover, pollen ontogeny is also an attractive model in which to dissect cellular networks that control cell growth, asymmetric cell division and cellular differentiation. Our objective, and an essential step towards the detailed understanding of these processes, was to comprehensively define the male haploid transcriptome throughout development. We have developed staged spore isolation procedures for Arabidopsis and used Affymetrix ATH1 genome arrays to identify a total of 13,977 male gametophyte-expressed mRNAs, 9.7% of which were male-gametophyte-specific. The transition from bicellular to tricellular pollen was accompanied by a decline in the number of diverse mRNA species and an increase in the proportion of male gametophyte-specific transcripts. Expression profiles of regulatory proteins and distinct clusters of coexpressed genes were identified that could correspond to components of gametophytic regulatory networks. Moreover, integration of transcriptome and experimental data revealed the early synthesis of translation factors and their requirement to support pollen tube growth. The progression from proliferating microspores to terminally differentiated pollen is characterized by large-scale repression of early program genes and the activation of a unique late gene-expression program in maturing pollen. These data provide a quantum increase in knowledge concerning gametophytic transcription and lay the foundations for new genomic-led studies of the regulatory networks and cellular functions that operate to specify male gametophyte development.
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影响因子：
5.1
作者：
Hennig, L;Menges, M;Gruissem, W
通讯作者：
Gruissem, W
影响因子：
6.9
作者：
Brandt, S;Kloska, S;Kehr, J
通讯作者：
Kehr, J
影响因子：
11.8
作者：
Chiang, MK;Melton, DA
通讯作者：
Melton, DA
影响因子：
7.4
作者：
Honys, D;Twell, D
通讯作者：
Twell, D
DOI：
10.1073/pnas.232590499
发表时间：
2002-11-26
影响因子：
11.1
作者：
Demura, T;Tashiro, G;Fukuda, H
通讯作者：
Fukuda, H