How much metagenomic sequencing is enough to achieve a given goal?
How much metagenomic sequencing is enough to achieve a given goal?
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多少宏基因组测序足以实现既定目标？
DOI：
10.1038/srep01968
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发表时间：
2013
影响因子：
4.6
通讯作者：
Yu, Yuhe
中科院分区：
文献类型：
--
作者：
Ni, Jiajia;Yan, Qingyun;Yu, Yuhe
宏基因组学研究极大地扩展了我们对微生物世界的了解。此外，随着高通量测序技术的发展，用于测序的样本量显着增加。然而，完全捕获环境中每种微生物携带的所有DNA序列仍然是不可能的。因此，估算一个合理、实用的测序量以实现目标就显得尤为必要。在本研究中，我们介绍了一种新的方法来估计所需的最小量的宏基因组测序为一个给定的目标。我们还计算了每个操作分类单元的基因组比例和特定基因的检测效率（我们使用SSU rRNA基因作为示例），基于随机宏基因组测序的给定量。在宏基因组研究中，合理、实用的测序量估算将为高通量测序提供参考。
Metagenomic studies have dramatically expanded our knowledge of the microbial world. Furthermore, the amount of sample for sequencing has significantly increased with the development of high-throughput sequencing technologies. However, fully capturing all DNA sequences carried by every microorganism in the environment is still impossible. Therefore, estimating a reasonable and practical amount for sequencing to achieve the objectives is particularly necessary. In the present study, we introduce a novel method for estimating the required minimum amount for metagenomic sequencing for a given goal. We also calculated the genomic proportion of each operational taxonomic unit and the detection efficiency of a specific gene (we have used SSU rRNA gene as an example) based on a given amount for random metagenomic sequencing. The reasonable and practical estimated amount for sequencing in metagenomic studies will provide good reference information when applying high-throughput sequencing for a given goal.
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