Integrated fluorescent cytology with nano-biologics in peritoneally disseminated gastric cancer.
Integrated fluorescent cytology with nano-biologics in peritoneally disseminated gastric cancer.
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DOI：
10.1111/cas.13760
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发表时间：
2018-10
期刊：
影响因子：
5.7
通讯作者：
Fujiwara T
中科院分区：
文献类型：
--
作者：
Watanabe M;Kagawa S;Kuwada K;Hashimoto Y;Shigeyasu K;Ishida M;Sakamoto S;Ito A;Kikuchi S;Kuroda S;Kishimoto H;Tomida S;Yoshida R;Tazawa H;Urata Y;Fujiwara T
腹膜细胞学阳性的胃癌患者肿瘤复发的风险增加，但尽管有一定比例的细胞学阳性患者迅速复发并伴有侵袭性进展，但其他患者在常规化疗中存活时间更长。这种异质性使得难以对患者进行更密集的治疗，并对精准医疗的实施提出了重大挑战。我们开发了一种新的方法来鉴定细胞学阳性胃癌患者中的生物学恶性亚群，使用表达绿色荧光蛋白（GFP）的减毒腺病毒，其中端粒酶启动子调节病毒复制（TelomeScan，OBP-401）。使用多模式酶标仪成功定量了TelomeScan阳性细胞发出的荧光。然后，我们分析了68例胃癌患者的临床腹膜冲洗液，发现端粒扫描阳性的患者预后明显较差。在21例细胞学阳性患者中，端粒扫描阳性患者的中位生存时间（235天）显著短于端粒扫描阴性患者（671天; P = 0.0062）。这种荧光病毒引导的细胞学检测胃癌患者腹膜冲洗液中的生物学恶性癌细胞，因此可能有助于基于播散细胞遗传特征的治疗分层和精准医学方法。
Gastric cancer patients positive for peritoneal cytology are at increased risk of tumor recurrence, but although a certain proportion of cytology‐positive patients relapse rapidly with aggressive progression, others survive longer with conventional chemotherapies. This heterogeneity makes it difficult to stratify patients for more intensive therapy and poses a substantial challenge for the implementation of precision medicine. We developed a new approach to identify biologically malignant subpopulations in cytology‐positive gastric cancer patients, using a green fluorescent protein (GFP)‐expressing attenuated adenovirus in which the telomerase promoter regulates viral replication (TelomeScan, OBP‐401). The fluorescence emitted from TelomeScan‐positive cells was successfully quantified using a multi‐mode microplate reader. We then analyzed clinical peritoneal washes obtained from 68 gastric cancer patients and found that patients positive for TelomeScan had a significantly worse prognosis. In 21 cytology‐positive patients, the median survival time of those who were TelomeScan positive (235 days) was significantly shorter than that for those who were TelomeScan negative (671 days; P = 0.0062). This fluorescent virus‐guided cytology detects biologically malignant cancer cells from the peritoneal washes of gastric cancer patients and may thus be useful for both therapy stratification and precision medicine approaches based on genetic profiling of disseminated cells.
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影响因子：
7.4
作者：
Isobe, Yoh;Nashimoto, Atsushi;Akazawa, Kohei;Oda, Ichiro;Hayashi, Kenichi;Miyashiro, Isao;Katai, Hitoshi;Tsujitani, Shunichi;Kodera, Yasuhiro;Seto, Yasuyuki;Kaminishi, Michio
通讯作者：
Kaminishi, Michio
影响因子：
7.4
作者：
Dalal, Kimberly Moore;Woo, Yanghee;Coit, Daniel G.
通讯作者：
Coit, Daniel G.
影响因子：
3.7
作者：
Bentrem, D;Wilton, A;Coit, D
通讯作者：
Coit, D
影响因子：
2.7
作者：
Kitayama J;Ishigami H;Yamaguchi H;Sakuma Y;Horie H;Hosoya Y;Lefor AK;Sata N
通讯作者：
Sata N
影响因子：
3
作者：
Iizumi, Sakura;Takashima, Atsuo;Boku, Narikazu
通讯作者：
Boku, Narikazu