Extensive Identification of Bacterial Riboflavin Transporters and Their Distribution across Bacterial Species.
Extensive Identification of Bacterial Riboflavin Transporters and Their Distribution across Bacterial Species.
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DOI：
10.1371/journal.pone.0126124
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
García-Angulo VA
中科院分区：
文献类型：
--
作者：
Gutiérrez-Preciado A;Torres AG;Merino E;Bonomi HR;Goldbaum FA;García-Angulo VA
核黄素是黄素单核苷酸(FMN)和黄素腺嘌呤二核苷酸(FMN)的前体，是所有生物体必需的代谢物。虽然核黄素从头合成和核黄素进口的功能可能在细菌中共存，但这种共存的程度尚不确定。对RibM、RibN、RfuABCD和能量偶联因子-Ribu细菌核黄素转运体进行了实验表征。此外，ImpX、RfnT和RibXY被认为是基于与核黄素生物合成途径(RBP)基因的位置聚类或保守的FMN核糖开关调控元件的核黄素转运体。在这里，我们在细菌基因组中寻找FMN核糖开关，以确定编码核黄素转运蛋白的基因，并评估它们在细菌中的分布。发现了两个新的核黄素转运蛋白：梭状芽胞杆菌中的RibZ和花中质体中的RibV。对一株大肠杆菌核黄素营养缺陷型菌株的反式互补实验证实了艰难梭菌的RibZ、橙色弯曲菌的RibXY、核梭杆菌的ImpX和人嗜铬杆菌的RfnT具有核黄素转运活性。对所有已知的细菌核黄素转运蛋白的基因组分布分析表明，大多数存在于拥有RBP的物种中，一些细菌甚至可能编码来自两个不同家族的功能性核黄素转运蛋白。我们的结果表明，一些物种拥有祖先的核黄素转运蛋白，而另一些物种拥有似乎是最近进化出来的转运蛋白。此外，我们的数据表明，也存在不明的核黄素转运体。目前的研究使实验表征的核黄素转运蛋白的数量增加了一倍，并表明这些蛋白在核黄素原营养细菌中具有特定的、非辅助的作用。
Riboflavin, the precursor for the cofactors flavin mononucleotide (FMN) and flavin adenine dinucleotide, is an essential metabolite in all organisms. While the functions for de novo riboflavin biosynthesis and riboflavin import may coexist in bacteria, the extent of this co-occurrence is undetermined. The RibM, RibN, RfuABCD and the energy-coupling factor-RibU bacterial riboflavin transporters have been experimentally characterized. In addition, ImpX, RfnT and RibXY are proposed as riboflavin transporters based on positional clustering with riboflavin biosynthetic pathway (RBP) genes or conservation of the FMN riboswitch regulatory element. Here, we searched for the FMN riboswitch in bacterial genomes to identify genes encoding riboflavin transporters and assessed their distribution among bacteria. Two new putative riboflavin transporters were identified: RibZ in Clostridium and RibV in Mesoplasma florum. Trans-complementation of an Escherichia coli riboflavin auxotroph strain confirmed the riboflavin transport activity of RibZ from Clostridium difficile, RibXY from Chloroflexus aurantiacus, ImpX from Fusobacterium nucleatum and RfnT from Ochrobactrum anthropi. The analysis of the genomic distribution of all known bacterial riboflavin transporters revealed that most occur in species possessing the RBP and that some bacteria may even encode functional riboflavin transporters from two different families. Our results indicate that some species possess ancestral riboflavin transporters, while others possess transporters that appear to have evolved recently. Moreover, our data suggest that unidentified riboflavin transporters also exist. The present study doubles the number of experimentally characterized riboflavin transporters and suggests a specific, non-accessory role for these proteins in riboflavin-prototrophic bacteria.
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影响因子：
6.4
作者：
Deka RK;Brautigam CA;Biddy BA;Liu WZ;Norgard MV
通讯作者：
Norgard MV
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DOI：
10.1093/bioinformatics/btt509
发表时间：
2013-11-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
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作者：
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通讯作者：
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影响因子：
3.5
作者：
Hemberger S;Pedrolli DB;Stolz J;Vogl C;Lehmann M;Mack M
通讯作者：
Mack M
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
DOI：
10.1073/pnas.1004716107
发表时间：
2010-07-20
影响因子：
11.1
作者：
Koepke, Michael;Held, Claudia;Duerre, Peter
通讯作者：
Duerre, Peter