Quantitative imaging of airway liquid absorption in cystic fibrosis.
Quantitative imaging of airway liquid absorption in cystic fibrosis.
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DOI：
10.1183/09031936.00220513
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发表时间：
2014-09
期刊：
影响因子：
--
通讯作者：
Corcoran TE
中科院分区：
文献类型：
--
作者：
Locke LW;Myerburg MM;Markovetz MR;Parker RS;Weber L;Czachowski MR;Harding TJ;Brown SL;Nero JA;Pilewski JM;Corcoran TE
New measures are needed to rapidly assess emerging treatments for cystic fibrosis (CF) lung disease. Using an imaging approach, we evaluated the absorptive clearance of the radiolabeled small molecule probe diethylene triamine penta-acetic acid (DTPA) as an in vivo indicator of changes in airway liquid absorption. DTPA absorption and mucociliary clearance rates were measured in 21 patients with CF (12 adults and nine children) and nine adult controls using nuclear imaging. The effect of hypertonic saline on DTPA absorption was also studied. In addition, in vitro studies were conducted to identify the determinants of transepithelial DTPA absorption. CF patients had significantly increased rates of DTPA absorption compared with control subjects but had similar mucociliary clearance rates. Treatment with hypertonic saline resulted in a decrease in DTPA absorption and an increase in mucociliary clearance in 11 out of 11 adult CF patients compared with treatment with isotonic saline. In vitro studies revealed that ~50% of DTPA absorption can be attributed to transepithelial fluid transport. Apically applied mucus impedes liquid and DTPA absorption. However, mucus effects become negligible in the presence of an osmotic stimulus. Functional imaging of DTPA absorption provides a quantifiable marker of immediate response to treatments that promote airway surface liquid hydration.
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DOI：
10.1183/09031936.00059009
发表时间：
2010-04
期刊：
The European respiratory journal
影响因子：
--
作者：
Corcoran TE;Thomas KM;Myerburg MM;Muthukrishnan A;Weber L;Frizzell R;Pilewski JM
通讯作者：
Pilewski JM
DOI：
10.1152/ajplung.00116.2012
发表时间：
2012-07-01
影响因子：
4.9
作者：
Li, Xiaopeng;Comellas, Alejandro P.;Zabner, Joseph
通讯作者：
Zabner, Joseph
DOI：
10.1165/rcmb.2009-0348oc
发表时间：
2010-12-01
影响因子：
6.4
作者：
Myerburg, Michael M.;Harvey, Peter R.;Butterworth, Michael B.
通讯作者：
Butterworth, Michael B.
DOI：
10.1513/pats.200703-042br
发表时间：
2007-08-01
期刊：
Proceedings of the American Thoracic Society
影响因子：
--
作者：
Donaldson, Scott H;Corcoran, Timothy E;Bennett, William D
通讯作者：
Bennett, William D
影响因子：
4.8
作者：
Eckford, Paul D. W.;Li, Canhui;Bear, Christine E.
通讯作者：
Bear, Christine E.